小麦基因组的一种简易提取方法

摘要：【研究目的】在综合分析多种提取小麦DNA方法的基础上，改良发展了一种快速简便高效的小麦基因组DNA提取方法。【方法】以春小麦Thatcher和以Thatcher为背景的近等基因系TcLr19，TcLr20， TcLr28为材料，改进后的方法提取的基因组DNA用抗叶锈基因Lr20的STS标记、Lr19和Lr28的SCAR标记、Lr28的SSR 标记进行PCR扩增，【结果】各分子标记都扩增出条带清晰正确的目的片段【结论】这表明该方法能够获得适用于STS、SCAR、SSR标记PCR扩增的高质量的DNA，而且该DNA简易提取方法加快了DNA提取速度、降低了污染源和成本，为大批量提取DNA提供了技术支撑。

One simple method for extracting of the wheat genome

One easy and efficient wheat genomic DNA extraction method had been developed and named as TENR extraction method. Spring wheat cultivar Thatcher, the near-isogenic lines TcLr19, TcLr20 and TcLr28 which taken the Thatcher as the genetic background were used as the materials and the DNA were extracted by TENR method. The quality of DNA were evaluated by UV spectrophotometric analysis, agarose gel electrophoresis method and PCR amplification using the STS marker for leaf rust resistance gene Lr20, SCAR markers for Lr19 and Lr28, SSR marker for Lr28. The results showed that high qualified DNA was obtained by TENR extraction method, clear and right bands were amplified in the test. The method could reduce the DNA extracting processing, costs, pollution to the environment, and get high quality DNA for PCR analysis. It provided base for the preparation of large number of samples for PCR-based assays.