白蚁肠道木质素分解菌、染料脱色菌的分离鉴定及产酶条件优化

分离具有木质素分解功能的菌株,并优化其产酶条件,同时观察分离菌株对染料的脱色情况,可为有效分解利用木质素提供必要的微生物资源。利用苯胺蓝平板法从黑胸散白蚁肠道中分离到2株具有木质素分解功能的菌株PY12、MX5,经16srRNA鉴定PY12、MX5分别为肠杆菌属的霍氏肠杆菌(Enterobacter hormaechei)、芽孢杆菌属的地衣芽孢杆菌(Bacillus licheniformis)。优化后菌株PY12LiP活力提高为278U/L,菌株MX5LiP活力提高为256U/L。并研究二者对6种染料的脱色效果观察,发现菌株PY12、MX5对6种染料都有不同程度的脱色作用,但对结晶紫脱色作用较弱。

Isolation,Identification and Optimization of Fermentation Conditions of Lignin Decomposition bacterium and Decolorization bacteria in Termite Gut

To provide necessary microbial resources for catalyzing lignin,the strains decomposing lignin were isolated and the fermentation conditions was optimized,the decolorizing effects of the isolated strains were investigated.The results showed that 2 strains,named PY12 and MX5,with decomposing lignin function were isolated using Azure B method from reticulitermes chinensis snyder gut.By 16 s rRNA identification,we found PY12 and MX5 belonged to Schizophyllum commune and Irpex lacteus,respectively.After optimizing the conditions of treatment to strains,the enzyme activities of PY12 and MX5 significantly increased to 278 U/L and 256 U/L,respectively.And PY12,MX5 showed some degree of decolorizing effects on six kinds of dyes,but they have a very weak decolorizing function to crystal violet.