PCR-ELISA法对大豆品种的转基因定性检测研究

以共价交联在PCR管壁上的寡核苷酸作为固相引物进行PCR扩增,对PCR扩增的固相和液相产物分别进行杂交和凝胶电泳检测的PCR-ELISA法,对黑龙江省常规选育品种合丰35和黑农37、外源DNA直接导入大豆的分子育种所获品种黑生101、及大连进口的美国2号等大豆,进行转基因定性检测.结果表明:该方法高效可靠,可作为一种快速定性检测转基因产品的方法;检测结果:美国2号为转基因大豆,黑生101、合丰35和黑农37为非转基因大豆.上述结果对分子育种、生态保护、安全监测及对建立黑龙江省非转基因大豆生产保护区等具有重要意义.

STUDY ON QUALITATIVE TEST OF TRANSGENES BY PCR-ELISA IN SOYBEAN CULTIVARS

The oliqonucletides which covalent primers, bonded on the wall of a PCR tube as solid primers, have bean amplified by PCR equipment. The Solid and Liquid phase amplified by PCR were hybridized and done with gel-eleitro phoresis respectively. Some Heilongjiang soybean cultivars, Suchas Hefeng 35 and Heinong 37(bred by conventional breeding), Heisheng 101(bred by introducing exogenous DNA through pollen tube channel, and Soybean of American 2 from Dalian importing, were tested by the PCR-ELISA. The result showed that Soybean of American 2 was a transgenic soybean, Heisheng 101, Hefeng 35 and Heinong 37 were not. Those have important meaning in plant molecular breeding, ecological protecting and safety monitoring.