| 大麦黄矮病毒GPV株系基因组末端序列的克隆和分析 |
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| 引用本文： | 张文蔚,成卓敏. 大麦黄矮病毒GPV株系基因组末端序列的克隆和分析[J]. 中国农业科技导报, 2009, 11(1): 102-107 |
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| 作者姓名： | 张文蔚 成卓敏 |
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| 作者单位： | （中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室, 北京 100193） |
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| 摘 要： | 利用5′RACE 、3′RACE和RT-PCR完成了大麦黄矮病毒GPV株系5′和3′末端序列的克隆和分析。分析
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| 关 键 词： | 大麦黄矮病毒GPV株系；5′RACE；3′RACE；5′UTR；3′UTR |
| 收稿时间： | 2008-09-24 |
| 修稿时间： | 2008-11-28 |
Cloning and Analysis of the Terminal Sequence of Barley Yellow Dwarf Virus GPV |
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| ZHANG Wen-wei,CHENG Zhuo-min. Cloning and Analysis of the Terminal Sequence of Barley Yellow Dwarf Virus GPV[J]. Journal of Agricultural Science and Technology, 2009, 11(1): 102-107 |
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| Authors: | ZHANG Wen-wei CHENG Zhuo-min |
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| Affiliation: | (State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection, ;Chinese Academy of Agricultural Sciences, Beijing 100193, China) |
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| Abstract: | The authentic 5′ and 3′ terminal sequences of genome of barley yellow dwarf virus(BYDV) GPV were obtained by 5′ and 3′ RACE and RT-PCR techniques. The analysis showedthat 5′ terminal sequence in the genome of GPV was 302 nt long, which included the startcodon ATG and 5′ un-translated region (UTR) of 100 nt. Compared with other virus ofPolerovirus, the length of 5′ UTR was variable and un-conservative. While 3′ terminalsequence in the genome of GPV was 328 nt long, including the terminator codon TGA and 3′UTR of 93 nt. 3′UTR was 74 nt shorter than that of RPV. The homology of 3′UTR betweenGPV and RPV was 40%, but the terminal of 3′UTR was conservative. The homology was 84.34%between GPV and RPV. |
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| Keywords: | 5'RACE 3'RACE 5'UTR 3'UTR |
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