Characterization of macrophage phagocytosis of the tropical fish Prochilodus scrofa (Steindachner, 1881) |
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| Affiliation: | 1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University, Huzhou 313000, China;2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Freshwater Fisheries Research Center, Wuxi 214081, China;1. Ocean College, Hebei Agricultural University, Qinhuangdao 066003, China;2. Life Sciences College, Cangzhou Normal University, Cangzhou 061001, China;1. Department of Infectious Diseases and Preventive Medicine, Veterinary Research Institute, Brno, Czech Republic;2. Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic;3. Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic;4. Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic;5. Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of AgriSciences, Mendel University in Brno, Czech Republic;6. Institute of Morphology, University of Veterinary Medicine, Vienna, Austria |
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| Abstract: | Measurements of in vivo and in vitro macrophage phagocytosis, using vital fluorescent dyes, were determined for Prochilodus scrofa in order to evaluate the effect of different stimuli on the health condition of this tropical fish. The Phagocytic Capacity (PC), Phagocytic Index (PI) and Germicide Capacity (GC) using Saccharomyces cerevisiae were estimated using intraperitoneal (i.p.) macrophages. In vivo macrophages (MØ) were stimulated following i.p. injections of the yeast, while in vitro measurements were made by harvesting i.p. phagocytes on coverslips and placing a yeast suspension over them. In vitro results show differences over time, with PC, PI and GC being highest at 7–14 days. In vivo results reveal few differences over time, and the highest PC, PI and GC being observed after 7 days. This work shows that in vitro MØ activation period and the in vivo yeast incubation period influence the macrophage phagocytic activity. For the first time, MØ phagocytosis of P. scrofa was characterised under light microscopy, transmission electron microscopy and scanning electron microscopy. |
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