大麦条纹花叶病毒(BSMV)在新疆的发生

 在新疆五家渠奇台黑芒小麦上分离到大麦条纹花叶病毒,致死温度60~65℃;体外存活期9~14天;稀释限点1:3000倍。人工接种可侵染禾本科植物,对接过种的非禾本科植物如灰藜、苋色藜、菠菜、甜菜、普通烟、心叶烟、枯斑三生烟、萹豆等不表现病状。用聚乙二醇沉淀和聚乙二醇沉淀、差速离心提取病毒,注入家兔体内,可形成相应的抗体,效价为1:128,1:1024,病毒颗粒为短棒状115~129×20毫微米。
初步观察,病株上的麦种带毒率与种子的饱满程度、品种、感病阶段有一定的相关性。病株上的花粉与健株母本杂交的种子不带毒。

OCCURENCE OF BARLEY STRIPE MOSAIC VIRUS IN XINJIANG

The barley stripe mosaic virus occurred and was isolated from wheat cv, chta-hemon in Wuji-aqu, Xinjiang.The isolate showed T.D.P.at 60-65℃ longevity in vitro 9-14 days and D.E.P. 1:3000. The virus was transmitted with mechanical inoculation, The host range was limited to the plant of gramineae. No symoptoms were shown on inoculated plants of Spinacia oleracea, Nicotiana glutinosa, N.tabacum, Sansum, Chenopodium amaranitcolor and Beta ulgaris.
The virus was purified by PEG and PEG-differential centrifugation and the partially purified virus was used as antigen for preparing antiserum.The antiserum produced reaches titres of 28:1 and 1:1024.The Percentage of seed transmission varied with wheat varieties and cultivars.
Results of tests on the pollen transmission were negative.