| 小麦叶片内几个抗冷相关基因cDNA片段的克隆 |
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| 引用本文： | 康国章,岳彩凤,沈丙权,刘超,王永华,郭天财. 小麦叶片内几个抗冷相关基因cDNA片段的克隆[J]. 河南农业科学, 2009, 0(5) |
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| 作者姓名： | 康国章 岳彩凤 沈丙权 刘超 王永华 郭天财 |
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| 作者单位： | 河南农楼下大学,国家小麦工程技术研究中心,河南,郑州,450002 |
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| 基金项目： | 中国博士后科学基金，河南省教育厅自然科学基金 |
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| 摘 要： | 运用RT-PCR法,从小麦叶片中克隆出SOD、CAT、APX、GR、CBF、GI等几个抗冷相关基因的cDNA片段,序列比对结果显示,这些基因与GenBank上已登录的大麦、小麦、玉米、水稻等相关基因的同源性较高,表明克隆出了上述几个小麦抗冷相关基因,其中的APX和GR为普通小麦中首次报道。
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| 关 键 词： | 小麦 抗冷基因 克隆 |
Cloning of Some Genes Related to Cold-tolerance in Wheat Leaves |
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| KANG Guo-zhang,YUE Cai-feng,SHEN Bing-quan,LIU Chao,WANG Yong-hua,GUO Tian-cai. Cloning of Some Genes Related to Cold-tolerance in Wheat Leaves[J]. Journal of Henan Agricultural Sciences, 2009, 0(5) |
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| Authors: | KANG Guo-zhang YUE Cai-feng SHEN Bing-quan LIU Chao WANG Yong-hua GUO Tian-cai |
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| Affiliation: | Engineering Research Center for Wheat;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | Partial cDNA sequences of superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),glutathione reductase(GR),C-repeat bing factor(CBF) and GIGANTEN(GI) were isolated from wheat leaves using RT-PCR.Sequence analysis showed that these genes had high identity to relevant genes from wheat,barley,maize and rice registered in GenBank,im-plying that these cloned fragments were really the cold-tolerant genes.Among them,APX and GR were firstly isolated from common wheat(Triticum aestivum L.). |
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| Keywords: | Triticum aestivum L. Cold-tolerant genes Cloning |
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