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龙爪槐提取物对铜绿微囊藻生长的抑制作用研究
引用本文:周晓见,夏洁,靳翠丽,缪莉,董昆明,封克. 龙爪槐提取物对铜绿微囊藻生长的抑制作用研究[J]. 农业科学与技术, 2011, 0(9): 1347-1349,1354
作者姓名:周晓见  夏洁  靳翠丽  缪莉  董昆明  封克
作者单位:扬州大学环境科学与工程学院;扬州大学海洋科学与技术研究所
基金项目:Supported by National Natural Science Foundation of China(41076097,41006097);Science and Technology Research Key Project of ChineseMinistry of Education(211065);Natural Science Foundation of Jiangsu Province,China(BK2010322);Open Research of Jiangsu Key Laboratory of Environmental Material and Environmental Engineering(K090027,K090025,K090026,K090028);Graduate Science and Technology Innovation Project of Department of Education,Jiangsu Province,China(M080960);"New Century"Talent Project of Yangzhou University,China~~
摘    要:[目的]探讨龙爪槐提取物对铜绿微囊藻生长的抑制作用。[方法]测定了龙爪槐叶片浸取液对铜绿微囊藻的增殖抑制效果,并对活性组分进行了初步研究和分析。[结果]龙爪槐叶片的无水乙醇浸提物经正己烷、乙酸乙酯、正丁醇和水依次分配后,提取物主要成分为极性组分(60%以上),而含量约为25%的非极性组分(正己烷相)具有显著的抑制铜绿微囊藻生长的效果。在25和50mg/L正己烷相组分作用下铜绿微囊藻的7d生长抑制率分别达75%和90%以上,同时还伴随着细胞中叶绿素a的破坏。[结论]为防治微囊藻水华提供了理论依据。

关 键 词:龙爪槐  铜绿微囊藻  正己烷相  叶绿素a

Study on the Inhibitory Effect of Sophora japonica Extracts against the Growth of Microcystis aeruginosa
ZHOU Xiao-jian,XIA Jie,JIN Cui-li,MIAO Li,DONG Kun-ming,FENG Ke. Study on the Inhibitory Effect of Sophora japonica Extracts against the Growth of Microcystis aeruginosa[J]. Agricultural Science & Technology, 2011, 0(9): 1347-1349,1354
Authors:ZHOU Xiao-jian  XIA Jie  JIN Cui-li  MIAO Li  DONG Kun-ming  FENG Ke
Affiliation:1,21.College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127;2.Marine Science and Technology Institute,Yangzhou University,Yangzhou 225127
Abstract:[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of M.aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated by n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority (>60%).The non-polar fraction in n-nexane (about 25%) was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theoretical basis for preventing and controlling the water bloom of M.aeruginosa.
Keywords:Sophora japonica  Microcystis aeruginosa  N-hexane phase  Chlorophyll a
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