池塘养殖患病泥鳅两种病毒病原的电镜观察初报

利用电子显微镜技术,对湖北荆州池塘养殖患病泥鳅的组织进行超薄切片电镜观察。电镜观察结果显示:在患病泥鳅的脾脏、肾脏、心脏及肠道组织中观察到大量球形和杆状两种形态的病毒颗粒。球形病毒颗粒无囊膜,直径约50~75 nm;杆状病毒颗粒有囊膜,形态多样,大小约为(50~100)nm×(300~500)nm;细胞培养分离病毒结果显示,球状病毒可在鲫鱼脑组织细胞系(Gi CB)中增殖,可导致细胞出现典型的细胞病变效应(CPE,cytopathic effect),但没有观察到Gi CB细胞中杆状病毒的增殖。

A preliminary observation of two viral pathogens in diseased pond-cultured loach(Misgurnus anguillicaudatus) by transmission electronic microscopy

A serious disease was occurred in the pond-cultured loach(Misgurnus anguillicaudatus) with high mortality in Jinzhou, Hubei, China.To figure the pathogen, the tissues of the diseased loach were sliced and subjected to transmission electron microscopy observation.The results showed that a number of spherical and rod-like viral particles were observed in the spleen, kidney, heart and gut tissue of the diseased loach.The spherical viral particles were non-enveloped, with a diameter of 50-75nm; While the rod-like viral particles were enveloped with a variety of shapes, and were about 50-100nm in diameter and about 300-500nm in length.Furthermore, the filtrates of the infected tissues were inoculated in gibel carp brain cells(GiCB).The results showed that the spherical virus could propagate and caused typical cytopathic effects(CPE) in GiCB cells.By contrast, the rod-like virus could not propagate in these cells.