岩原鲤MSTN基因克隆及饥饿对MSTN表达的影响

运用同源克隆的方法获得岩原鲤(Procypris rabaudi(Tchang))肌肉生长抑制素(myostatin,MSTN)c DNA全长序列,并通过逆转录PCR(RT-PCR)检测MSTN基因的组织差异性表达以及饥饿胁迫对MSTN基因表达的影响。结果显示,岩原鲤MSTN基因1 547 bp的c DNA序列,编码区为1 128 bp,编码375个氨基酸。PCR检测显示,该基因在肌肉和脑组织中大量表达,在眼、肠、心、鳃、肾和头肾组织中少量表达,在肝胰脏、脾脏组织中未见表达。饥饿再投喂实验表明肌肉组织中MSTN基因表达含量随着饥饿时间的延长逐渐升高,恢复投喂后3d表达含量急剧下降,投饵后6 d升至正常水平。结果表明,MSTN的表达对营养摄入敏感,在饥饿状态下岩原鲤通过上调MSTM表达来抑制肌肉生长,节约能量消耗。

Myostatin cDNA cloning and effect of starvation on mRNAs expression of MSTN in Procypris rabaudi( Tchang)

Myostatin cDNA complete nucleotide sequences of Procypris rabaudi(Tchang)was obtained by homology-based cloning methods in this study.Meanwhile, the expression of MSTN gene in different tissues and starvation stress were detec-ted by RT-PCR.The current study cloned a 1 547 bp cDNA of myostatin(MSTN)from P.rabaudi(Tchang).Its open read-ing frame(ORF)was 1 128 bp encoding 375 amino.RT-PCR analysis demonstrated that MSTN were extensively expressed in tissues of P.rabaudi with high level in the muscle and brain, modest level in the eye, gut, heart, gill, kidney and head kidney, but no expression in the hepatopancreas and spleen.Starvation refeeding experiments showed that MSTN ex-pression in muscle tissue of P.rabaudi gradually increased with the prolongation of starvation time.The expression of MSTN decreased sharply to the lowest level at 3 days after refeeding, and the expression increased to normal after 6 days.The re-sults suggested that the expression of MSTN was sensitive to nutrient intake, and ed s muscle growth was suppressed by up-regulating the expression of MSTM in order to save energy consumption in the starvation state.