| 唐菖蒲离体直接再生技术的研究 |
| |
| 引用本文： | 张智乾,何秀丽,义鸣放.唐菖蒲离体直接再生技术的研究[J].华中农业大学学报,2008,27(1):110-116. |
| |
| 作者姓名： | 张智乾 何秀丽 义鸣放 |
| |
| 作者单位： | 中国农业大学农学与生物技术学院,北京,100094 |
| |
| 摘 要： | 以唐菖蒲品种Advanced red的籽球、无菌根为外植体,研究了不同植物生长激素种类和浓度、硝酸银、籽球切割方式等因素对唐菖蒲离体直接再生的影响.结果表明:籽球切片直接再生植株的能力最强,无菌根次之.籽球切片直接诱导不定芽再生的最佳培养基为MS 2,4-D 0.6 mg/L 6-BA 1.1 mg/L AgNO3 1.5mg/L,不定芽再生率为85.95%,平均再生芽数为14.10;籽球接种方式以横切为宜,正反面对不定芽再生均无影响,而纵切接种只产生不定根;最佳生根培养基为MS IBA 0.5 mg/L,生根率达到91.81%,单苗根数为18.97.
|
| 关 键 词： | 唐菖蒲 籽球 无菌根 组织培养 直接再生 |
| 文章编号： | 1000-2421(2008)01-0110-07 |
| 修稿时间： | 2007年8月15日 |
Plant Direct Regeneration of Gladiolus in vitro |
| |
| ZHANG Zhi-qian HE Xiu-li YI Ming-fang.Plant Direct Regeneration of Gladiolus in vitro[J].Journal of Huazhong Agricultural University,2008,27(1):110-116. |
| |
| Authors: | ZHANG Zhi-qian HE Xiu-li YI Ming-fang |
| |
| Institution: | College of Agronomy and Biotechnology;China Agricultural University;Beijing 100094;China |
| |
| Abstract: | Cormlet and virus-free root of Gladiolus cv.advanced red with different cutting means were cultured on media with different plant growth substances and AgNO3.The results showed that plant direct regeneration rate of cormlet was much higher than that of virus-free root.The optimum medium for direct regeneration of adventitious buds from the cormlet was MS+2,4-D 0.6 mg/L+6-BA 1.1 mg/L+AgNO3 1.5 mg/L,in which the regeneration percentage of adventitious buds was 85.95%,and average adventitious buds was 14.10.Tr... |
| |
| Keywords: | Gladiolus cormlet virus-free root tissue culture direct regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《华中农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华中农业大学学报》下载免费的PDF全文 |
