Application of low‐frequency sonophoresis and reduction of antibiotics in the aquatic systems |
| |
| Authors: | C Cobo Labarca J Radinger V Schöning R Ariav R Jung K D Thompson W Kloas K Knopf |
| |
| Institution: | 1. Department of Ecophysiology and Aquaculture, Leibniz‐Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany;2. Faculty of Life Sciences, Albrecht Daniel Thaer‐Institute of Agricultural and Horticultural Sciences Humboldt University of Berlin, Berlin, Germany;3. Department of Fish Biology and Ecology, Leibniz‐Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany;4. Aqua‐Vet Technologies Ltd., Zichorn Ya'akov, Israel;5. BANDELIN Electronic GmbH & Co. KG, Berlin, Germany;6. Moredun Research Institute, Penicuik, Edinburgh, UK;7. Faculty of Life Sciences, Institute of Biology, Humboldt University, Berlin, Germany |
| |
| Abstract: | A major concern in aquaculture is the use of chemical therapeutics, such as antibiotics, because of their impact on the environment as well as on the fish product. As a potential tool for reducing antibiotic use, we tested the application of low‐frequency ultrasound as a method for enhancing antibiotic uptake. Rainbow trout juveniles (Oncorhynchus mykiss) were exposed to two different concentrations of oxytetracycline (OTC), flumequine (FLU) and florfenicol (FLO), administered by bath after the application of ultrasound. After exposure, concentrations of these substances were measured in the liver and blood of treated fish. Results showed that the ultrasound treatment can significantly increase the uptake for all three antibiotics. The uptake of OTC for example, in fish exposed to an OTC concentration of 20 mg L?1 after prior treatment with ultrasound, was similar to the OTC concentrations in their liver and blood to fish exposed to 100 mg L?1 without sonication. For FLU and FLO, the use of ultrasound caused significant differences of uptake in the liver at high antibiotic concentrations. This suggests that the use of ultrasound as a technique to deliver antibiotics to fish can ultimately reduce the amount of antibiotics discharged into the aquatic environment. |
| |
| Keywords: | antimicrobial environment microbiology rainbow trout sonication ultrasound |
|
|
