BnaYUCCA10基因在不同油酸含量油菜中的表达

为明晰油菜BnaYUCCA10基因与油酸含量之间的关系,以miRNA测序得到的BnaYUCCA10基因为对象,采用实时荧光定量PCR(RT–PCR)技术研究BnaYUCCA10基因在30份不同油酸含量的甘蓝型油菜不同部位和生长时期的表达情况,并对BnaYUCCA10基因是否参与油酸的代谢进行分析.结果表明:营养生长期大...

Expression of BnaYUCCA10 gene in Brassica napus with different oleic acid content

In order to clarify the relationship between the BnaYUCCA10 gene expression and the oleic acid content in Brassica napus, we used miRNA sequencing and real-time fluorescent quantitative PCR(RT-PCR) methods in this study to analyze the expression of BnaYUCCA10 gene in 30 samples collected from different tissues and growth periods of B. napus with different oleic acid content. And whether the oleic acid is involved in the metabolism was also analyzed. The results showed that the expression level of BnaYUCCA10 gene increased gradually with the growth process in most B. napus materials during the vegetative growth stage, and there was no significant difference between high oleic acid materials and non-high oleic acid materials. The expression level of 1 DAF flowers was higher than that of buds and 5 DAF flowers, and the expression level of high oleic acid materials and non-high oleic acid materials was significantly different. The expression level in silique stage decreased gradually with the growth process, and the expression level of high oleic acid materials and non-high oleic acid materials was significantly different. The expression level of the BnaYUCCA10 gene in silique stage and 5 DAF flowers was significantly related to the oleic acid content. The expression level of the high oleic acid materials was significantly higher than that in non-high oleic acid materials. It was suggested that BnaYUCCA10 gene might be involved in oleic acid metabolism in seeds, which could be of insight to the further research on the regulation mechanism of oleic acid in rapeseed.