Relationships between an Italian Strawberry Ecotype and its Ancestor using RAPD Markers |
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Authors: | Luigi Milella Danilo Saluzzi Mauro Lapelosa Giuseppe Bertino Piero Spada Ivana Greco Giuseppe Martelli |
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Institution: | (1) Dipartimento di Biologia, Difesa e Biotecnologie Agro-Forestali, Universitá della Basilicata, Potenza, Italy;(2) Ufficio Agricoltura, Comune di Maletto, Maletto, Catania, Italy;(3) Assessorato Agricoltura, UP 75 Regione Sicilia, Italy |
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Abstract: | Random-amplified polymorphic DNA (RAPD) markers were used to evaluate genetic variability among populations of an Italian
strawberry ecotype, and to determinate genetic relationships between genotypes and their putative ancestor. A total of 65
selections and one cultivar ‘Madame Moutot’ (MM), were analysed to evaluate genetic variability present in Etna mountain area
and to confirm as MM was one of the cultivars that originated the ecotype. A total of 222 RAPD markers was obtained using
16 decamer primers and 6 longer primers, 90.8% of the markers obtained by selected primers resulted polymorphic at least within
analysed genotypes. RAPDs were used to calculate genetic similarity coefficients and to generate dendrograms representing
genetic relationships among genotypes analysed. Cluster analysis displays as RAPD polymorphisms were able to characterize
the genotype variability among closely related groups. The data show as MM could be considered the ancestral genotypes introduced
in that area. The results obtained confirm that RAPD markers could be used as reliable markers to perform phylogenetic studies
in Fragaria×ananassa Duch. ex Rozier.
Giuseppe Bertino and Piero Spada - Coauthor involved in genotype selection and field management |
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Keywords: | Fragaria×ananassa Genetic variability Madame Moutot RAPDs |
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