| HRAP基因诱导型植物表达载体的构建及烟草转化 |
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| 引用本文： | 王爱英,缪建锟,彭晓明,沈海涛,祝建波.HRAP基因诱导型植物表达载体的构建及烟草转化[J].西北农业学报,2009,18(6):157-160. |
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| 作者姓名： | 王爱英 缪建锟 彭晓明 沈海涛 祝建波 |
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| 作者单位： | 石河子大学,生命科学学院,石河子大学农业生物技术重点实验室,石河子,832003 |
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| 基金项目： | 国家自然基金，国家转基因重大专项，石河子大学高层次人才启动项目，石河子大学科学研究发展计划"动植物育种专项计划项目" |
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| 摘 要： | 系统获得性抗性是植物抵御病菌侵染的一种独特的防御机制.HRAP基因是一种从甜胡椒中克隆的蛋白质激发子,可以有效引发植物系统性抗性.SAR8.2b基因是烟草系统获得性抗性信号传导途径中下游响应基因,受病原物和水杨酸诱导.本试验根据Genebank发布SAR8.2b基因的启动子序列,利用PCR技术,克隆了SAR8.2b基因的完整启动子,命名为SAR8.2bP,然后将SAR8.2bP构建到pUC18-HRAP载体中命名为pUC18-SAR8.2bP-HRAP,然后用SAR8.2bP-HRAP替换p2300-121载体中的35S启动子和GUS基因,重组子命名p2300-SAR8.2bP-HRAP.将构建的植物表达载体转化农杆菌GV3101,利用叶盘法转化烟草,通过PCR筛选,获得了转基因植株.为进一步验证转基因烟草的抗病效果奠定了基础.
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| 关 键 词： | HRAP基因 SAR8.2b启动子 系统获得性抗性 转基因烟草 |
| 收稿时间： | 2009/3/20 0:00:00 |
| 修稿时间： | 2009/4/24 0:00:00 |
Construction of Inducible Plant Expression Vector of HRAP Gene and Transform Tobacco |
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| WANG Aiying,MIAO Jiankun,PENG Xiaoming,SHEN Haitao and ZHU Jianbo.Construction of Inducible Plant Expression Vector of HRAP Gene and Transform Tobacco[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(6):157-160. |
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| Authors: | WANG Aiying MIAO Jiankun PENG Xiaoming SHEN Haitao and ZHU Jianbo |
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| Institution: | Biology College, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi Xinjiang 832003, China;Biology College, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi Xinjiang 832003, China;Biology College, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi Xinjiang 832003, China;Biology College, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi Xinjiang 832003, China;Biology College, Key Laboratory of Agriculture Biotechnology of Shihezi University, Shihezi Xinjiang 832003, China |
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| Abstract: | In the co-evolution with pathogens,plants have evolved complex integrated defense mechanisms against pathogens. Systemic acquired resistance is an important component of plant disease resistance mechanisms. HRAP is a elicitor which cloned from sweet pepper. It could trig plant SAR efficiently. SAR8. 26 was cloned gene was at downstream of tobacco SAR transduction pathway . The promoter of SAR8. 26, which could inducible by SA and pathogens. The SAR8. 26P was constructed to pUC18-HRAP,then replaced the 35S promoter and GUS by SAR8. 2bP-HRAP. The inducible plant expression vector was named for p2300-SAR8. 26P-HRAP. The recombine vector was transferred into Gv3101. The tobacco was transformed by leaf-disc method. Transgenic plants were identified by PCR. All of this will make the foundation of plant anti-diseases. |
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| Keywords: | HRAP gene SAR8 26 promoter Systemic acquired resistance Transgenic tobacco |
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