家兔胚胎细胞核移植的研究

兔超排后收集卵母细胞和１６细胞胚，以Ｍｃ－Ｇｒａｔｈ－Ｓｏｌｔｅｒ和Ｗｉｌｌａｄｓｅｎ两种去（注）核方法，将１６细胞胚细胞核经电融合植入去核成熟卵母细胞内，经体外培养或中间受体培养，使移核胚发育．结果表明：（１）兔胚核移植中上述两种去（注）法的成功率无差异；（２）ｈｃＧ注射后１３～１５ｈ，６７．８％的卵母细胞保留有第一极体；（３）强度为０．６３ｋＶ／ｃｍ，持续１６０μｓ的一次电脉冲可使７０．８％的注核胚融合，同样的电脉冲刺激卵母细胞的活化率为６１．１％；（４）兔移核胚在中间受体内或体外均可发育，在中间受体内有２３．０％可以发育到桑椹胚或囊胚．

STUDIES ON NUCLEAR TRANSPLANTATION IN RABBIT EMBRYOS

Superovulation in the does was conducted to obtain M Ⅱ oocytes and 16 cell embryos.Nuclei from 16 cell embryos were transplanted into enucleated oocytes throughelectrofusion,and by either McGrath Solter or Willadsen method the nuclear transplant(NT) embryos were cultured either in vitro or in the oviduets of the intermediatc recipientdoes.The results were as follows:(1)There was no signifiant difference(P>0.05)betweenMcGrath Solter and Willadsen methods in the success rate of manipulation duringenucleation and blastomere transfer.(2)67.8% of MⅡooeytes 13～15h post hCG showedfirst polar bodies.(3)A fusion rate of 70.8% of oocyte blastomere pairs was obtained withsingle pulses of 0.63kV/ cm lasting for 160μs and 61.1% of oocyes treated by the same pulseconditions were activated.(4)The NT embryos initiated development both in vitro and inthe oviduets of the intermediate recipients.23.0% of the NT embryos developed into morulacor blastocysts after culture in the intermediate recipicnts.