雪莲果中绿原酸含量的测定

目的]为雪莲果的开发利用提供理论依据。方法]称取10．0g雪莲果，洗净、切碎，加入40％的乙醇180ml，回流提取其中的绿原酸，自然冷却后过滤。波谱定性检验选择适宜的测定波长，以95％乙醇为参比溶液，用配制好的一系列不同浓度的绿原酸标准溶液绘制标准曲线，测定提取液的吸光度，计算滤液中绿原酸的含量。结果]绿原酸标准品和雪莲果样品提取液在330nm处有特征吸收峰，故选定330nm为最大吸收波长，测定雪莲果样品液的吸光度。雪莲果中绿原酸的平均吸光度值为1．105，含量为0．052％。采用分光光度法测得雪莲果中绿原酸的RSD值为3．07％，平均回收率为105．85％。结论]雪莲果中绿原酸的含量为0．052％。

Determination on Chlorogenic Acid Content in Smallanthus sonchifolius

Objective] The study was to provide the theoretical basis for development and utilization of Smallanthus sonchifolius.Method] 10.0 g S.sonchifolius was taken and the chlorogenic acid in it was extracted by 40% ethanol at 180 ml after it was washed and shreded,and the extracting solution was filtered after natural cooling.The suitable detection wavelength was selected through qualitative determination of wave spectrum.With 95% ethanol as the reference solution,the standard curve was drawed with a series of...