| 栽培种花生EST-SSR引物的开发及应用 |
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| 引用本文： | 肖洋,廖伯寿,晏立英,黄家权,任小平,雷永,姜慧芳.栽培种花生EST-SSR引物的开发及应用[J].湖北农业科学,2010,49(11). |
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| 作者姓名： | 肖洋 廖伯寿 晏立英 黄家权 任小平 雷永 姜慧芳 |
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| 作者单位： | 中国农业科学院油料作物研究所/油料作物生物学重点开放实验室,武汉,430062 |
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| 基金项目： | 国家"863"计划项目，国家自然科学基金 |
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| 摘 要： | 从高油抗青枯病花生新品系"06-4104"构建的5个cDNA文库获得了63207条EST,经序列拼接,获得14547条Uni-EST。经MISA检索,在这些Uni-EST中共检测出2643个SSR位点,分布于2250条EST中,发生频率为15.5%,平均每3.1kbEST序列含有一个SSR位点。其中,三核苷酸重复类型出现频率最高,占SSR总数的49.1%,其次是二核苷酸重复类型,占SSR总数的45.8%。在发现的46类重复基序中,AG/TC重复基序的频率最高,AAG/TTC次之。利用Primer3从含有SSR的2250条EST中共设计引物100对,利用这些引物检测花生栽培品种的多态性。结果表明,在所设计的100对引物中有91对在供试的6个花生栽培品种中得到有效扩增,其中13对得到了多态性的扩增产物,每对引物检测出的等位基因数2～3个,平均2.2个。
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| 关 键 词： | 花生 EST-SSR 标记开发 |
Development and Utilization of EST-SSR Primers in Peanut(Arachis hypogaea L.) |
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| XIAO Yang,LIAO Bo-shou,YAN Li-ying,HUANG Jia-quan,REN Xiao-ping,LEI Yong,JIANG Hui-fang.Development and Utilization of EST-SSR Primers in Peanut(Arachis hypogaea L.)[J].Hubei Agricultural Sciences,2010,49(11). |
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| Authors: | XIAO Yang LIAO Bo-shou YAN Li-ying HUANG Jia-quan REN Xiao-ping LEI Yong JIANG Hui-fang |
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| Institution: | XIAO Yang,LIAO Bo-shou,YAN Li-ying,HUANG Jia-quan,REN Xiao-ping,LEI Yong,JIANG Hui-fang(Oil Crop Research Institute/Key Lab for Biology of Oil Crops,Chinese Academy of Agricultural Sciences,Wuhan 430062,China) |
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| Abstract: | A total of 63 207 ESTs were produced from 5 cDNA libraries of RNAs of 06-4104,a peanut(Arachis hypogaea L.) line with high oil content and resistance to bacterial wilt.A total of 2 643 SSRs were identified,distributed in 2 250 ESTs.On average,one SSR was contained in 3.1 kb EST sequence with tri-nucleotide motifs(49.1%) as the most abundant motif types followed by di-nucleotide motifs(45.8%).AG/TC motifs' frequency is highest with AAG/TTC the next in 46 motifs found.Based on the 2 250 SSR-EST,100 primer pai... |
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| Keywords: | peanut EST-SSR marker development |
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