Comparison of Hypothermic and Normothermic Spinal Cord Perfusion in the Dog |
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| Authors: | STEVEN F SWAIM DVM MS MARC VANDEVELDE DVM † WILLIAM C SAMMONS DVM ¶ LINDA BAINE DVM § JOHN A McGUIRE PhD ‡ |
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| Institution: | Department of Small Animal Surgery and Medicine, School of Veterinary Medicine, Auburn University, Auburn, Alabama;*Department of Small Animal Surgery and Medicine, School of Veterinary Medicine, Auburn University, Auburn, Alabama.;?Institute for Comparative Neurology, University of Bern, Switzerland.;¶Academy Animal Hospital, Ft. Lauderdale, Florida.;§Bay Animal Hospital, Panama City, Florida.;?Research Data Analysis Department, Auburn University. |
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| Abstract: | Lumbar dorsal laminectomies were performed on 24 dogs, and exposed spinal cords were subjected to various time-temperature combinations of perfusion according to the following schedule: 1) 30 minutes of hypothermic perfusion—6 dogs; 2) 30 minutes of normothermic perfusion—6 dogs; 3) 120 minutes of hypothermic perfusion—6 dogs; and 4) 120 minutes of normothermic perfusion—6 dogs.
Surgery, cord perfusion, and exposure of the dorsal aspects of the spinal cord to the epaxial musculature for 5 days following surgery produced histopathologic changes in the dorsal aspects of the cord, especially around the dorsal root area. Hypothermic spinal cord perfusion for 120 minutes produced a marked deleterious effect on normal spinal cord function and spinal cord tissue. Spinal cords exposed to 30 minutes of hypothermic or normothermic perfusion or 120 minutes of normothermic perfusion had less severe clinical and histopathologic changes than those exposed to 120 minutes of hypothermic perfusion. Hypothermic perfusion for 30 minutes and 120 minutes affected not only dorsal aspects of the cord, but also the grey matter. The most severe change in grey matter occurred in cords perfused for 120 minutes. |
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