Data to the sex determination in <Emphasis Type="Italic">Pistacia</Emphasis> species using molecular markers |
| |
Authors: | B?Esfandiyari G?H?Davarynejad F?Shahriari M?Kiani Email author" target="_blank">A?MatheEmail author |
| |
Institution: | 1.Department of Horticulture,Ferdowsi University of Mashhad,Mashhad,Iran;2.Department of Plant Breeding and Biotechnology,Ferdowsi University of Mashhad,Mashhad,Iran;3.Research Center for Plant Sciences,Ferdowsi University of Mashhad,Mashhad,Iran;4.Faculty of Agriculture and Food Science,University of West Hungary,Mosonmagyaróvár,Hungary |
| |
Abstract: | Sex identification in Pistacia species during the long juvenile stage is an economically desirable objective. Due to the lack of morphological methods to
identify sex at this stage, the application of molecular markers is expected to facilitate breeding programs. The aim of our
study was to identify a marker closely linked to sex loci in Pistacia
atlantica Desf subsp. mutica, P. khinjuk, and P. vera subsp. Sarakhs. Samples were collected from both male and female plants of each species, and their band patterns were analyzed according
to the presence or absence of specific bands. Thirty random amplified polymorphic DNA (RAPD) primers and a pair of sequence
characterized amplified region (SCAR) primers were tested as potential markers of sex in wild Pistacia species. Among the RAPD primers, only BC1200 was found to amplify a specific sex band present in female plants. Based on our analysis of all individual samples, a fragment
of approximately 300 bp was amplified in female trees but absent in male ones. Although sex determination mechanisms in Pistacia are still unknown, they may be controlled by a single locus that acts as a trigger. The SCAR technique has proved to be a
reliable technique in gender determination of pistachio genotypes at the seedling phenophase. This method could reduce both
the time and costs associated with breeding programs. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|