| 重金属镉抗性基因czcA原核载体构建与鉴定 |
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| 引用本文： | 窦敏娜.重金属镉抗性基因czcA原核载体构建与鉴定[J].陕西农业科学,2023,69(7):8-10. |
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| 作者姓名： | 窦敏娜 |
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| 作者单位： | （咸阳职业技术学院，陕西 咸阳 712000） |
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| 摘 要： | 首先，将含有强抗镉czcA基因的重组质粒pGM-T载体进行PCR扩增和鉴定，其次，将带有双酶切位点的czcA基因与pGEX-4T-2载体构建，转化于E.coli BL21 DE3中，最后，经PCR鉴定和测序检测均表明试验成功，为下一步czcA基因片段进行诱导融合蛋白的表达提供了前提。
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| 关 键 词： | 镉 czcA pGEX-4T-2 构建 |
Prokaryotic Vector Construction and Identification of Cadmium Resistance Gene |
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| DOU Minna.Prokaryotic Vector Construction and Identification of Cadmium Resistance Gene[J].Shaanxi Journal of Agricultural Sciences,2023,69(7):8-10. |
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| Authors: | DOU Minna |
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| Institution: | (Xianyang Vocational and Technical College, Xianyang,Shaanxi 712000, China) |
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| Abstract: | In this study, we aimed to construct a prokaryotic vector for the expression of the Cadmium Resistance Gene (czcA).CzcA was amplified from the plasmid of pGM-T by PCR, and subsequently inserted into pGEX-4T-2 vector using a restriction enzyme. By agrose gel electrophoresis and DNA sequencing, czcA gene was induced into pGEX-4T-2 successfully. |
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