| PRRSV、CSFV和JEV混合感染的多联PCR诊断 |
| |
| 引用本文： | 陈钟鸣,张定东,方光远,张志成,张智勇.PRRSV、CSFV和JEV混合感染的多联PCR诊断[J].动物医学进展,2008,29(12). |
| |
| 作者姓名： | 陈钟鸣 张定东 方光远 张志成 张智勇 |
| |
| 作者单位： | 金陵科技学院,江苏,南京,210038;河南科技学院,河南,新乡,463000 |
| |
| 基金项目： | 江苏省高校科研成果推进项目(06KJD230065);;金陵科技学院基金资助项目 |
| |
| 摘 要： | 通过对猪繁殖与呼吸综合征病毒、猪瘟病毒、乙型脑炎病毒等的基因序列分析，设计多联PCR诊断方法诊断发病猪场上述病毒的混合感染，发现单病毒感染率在68．79／6～81．5％之间，混合感染率在58．9％～90％之间。采取的防制措施是研制和普遍使用新型疫苗，严防病原体传入，扑杀危险患病猪只，封闭式饲养等综合性措施，获得了较好的效果。
|
| 关 键 词： | 猪繁殖与呼吸综合征 猪"高热病" 多联PCR |
Multiplex PCR Diagnosis of Mixed Infection of PRRSV, CSFV and JEV |
| |
| CHEN Zhong-ming,ZHANG Ding-dong,FANG Guang-yuan,ZHANG Zhi-cheng,ZHANG Zhi-yong.Multiplex PCR Diagnosis of Mixed Infection of PRRSV, CSFV and JEV[J].Progress In Veterinary Medicine,2008,29(12). |
| |
| Authors: | CHEN Zhong-ming ZHANG Ding-dong FANG Guang-yuan ZHANG Zhi-cheng ZHANG Zhi-yong |
| |
| Institution: | 1.Jinling Institute of Technology;Nanjing;Jiangsu;210038;China;2.Henan Institute of Science and Technology;Xinxiang;Henan;463000;China |
| |
| Abstract: | By sequence analysis of PRRSV,CSFV,JEV and other viruses,multiple PCR was well designed to detect various viruses in mixed infection.The results indicated that infection rate of single pathogen was 68.7%-81.5%,while mixed infection rate was 58.9%-90%.Furthermore,the prevention and control measures,such as using new vaccine,preventing pathogen introduction,killing pigs suffered from illness,and closed management,were proposed. |
| |
| Keywords: | PRRS swine fever multiplex PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
