| 利用ISSR分子标记检测老化扁蓿豆种质遗传完整性的变化 |
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| 引用本文： | 王小丽,李志勇,李鸿雁,师文贵.利用ISSR分子标记检测老化扁蓿豆种质遗传完整性的变化[J].草原与草坪,2010,30(6):84-88. |
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| 作者姓名： | 王小丽 李志勇 李鸿雁 师文贵 |
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| 作者单位： | 甘肃农业大学草业学院草业生态系统教育部重点实验室;中国农业科学院草原研究所农业部沙尔沁牧草资源重点野外科学观测试验站; |
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| 基金项目： | 农业部农作物种质资源保护与利用项目，牧草种质资源保护项目 |
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| 摘 要： | 采用ISSR分子标记,对经老化处理的不同发芽率水平扁蓿豆种质进行遗传完整性分析。结果表明:用10个有效引物对4个不同发芽率居群进行PCR扩增,共检测到48个等位基因,每个位点的等位基因数为3～8个,平均为4.8个;老化处理群体的多态性位点百分率(PPB)、等位基因数(Na)、有效等位基因数(Ne)、基因多样性指数(H)、Shannon指数(I)等遗传参数,与对照群体的遗传参数相比都有所下降,表明老化处理后群体内的遗传多样性低于对照群体的遗传多样性;与对照群体相比,发芽率为20%时,各遗传参数呈极显著性差异,说明扁蓿豆种质更新发芽率临界值在20%～40%;ISSR分子标记可以作为老化扁蓿豆种子遗传完整性变化的检测方法,同时认为对于异质种质资源材料,低发芽率更新标准不利于种质资源遗传完整性的保持。
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| 关 键 词： | 扁蓿豆 种子老化 遗传完整性 ISSR 种质资源保存 |
Effect of seed aging treatment on changes of genetic integrity in Medicago ruthenica germplasm |
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| WANG Xiao-li,LI Zhi-yong,LI Hong-yan,SHI Wen-gui.Effect of seed aging treatment on changes of genetic integrity in Medicago ruthenica germplasm[J].Grassland and Turf,2010,30(6):84-88. |
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| Authors: | WANG Xiao-li LI Zhi-yong LI Hong-yan SHI Wen-gui |
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| Institution: | WANG Xiao-li1,2,LI Zhi-yong2,LI Hong-yan2,SHI Wen-gui2 (1. Key Laboratory for Grassland Ecosystems,Ministry of Education,Pratacultural College of Gansu Agricultural University,Lanzhou 730070,China,2. Key Prognosticate and Research Station of Grass Resources in Shaerqin,Ministry of Agriculture,Grassland Research Institute,Chinese Academy of Agriculture Sciences,Hohhot 010010,China) |
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| Abstract: | The effects of artificial aging treatments on genetic integrity of Medicago ruthenica germplasm with different germination rates were studied by using ISSR markers.The results showed that 48 alleles were detected, number of alleles per locus was between 3 to 8,mean number of alleles was 4.8 with 10 primers amplification by PCR;the percentage of polymorphic bands,number of alleles(Na),effective number of alleles(Ne),Nei's gene diversity index(H)and Shannon's information index(I) were lower in artificially ag... |
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| Keywords: | Medicago ruthenica seed aging genetic integrity ISSR germplasm conservation |
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