一株假单胞菌的分离鉴定及其在青海地区堆肥中的应用潜力

为了筛选出适用于青海地区油菜秸秆纤维素降解的菌株,提高当地油菜秸秆堆肥的效果,从青海省西宁市北山土壤中分离得到1株纤维素降解菌,经鉴定为假单胞菌属(Pseudomonas sp.),其羧甲基纤维素(CMC)酶活为25.35 U·mL-1,外切-β-葡聚糖酶活为22.33 U·mL-1,滤纸酶活为20.58 U·mL-1...

Isolation and identification of a Pseudomonas strain and its application potential in rape straw composting in Qinghai,China

In order to accelerate the cellulose degradation of rape straw and improve the composting quality in Qinghai, China, a cellulose degrading strain was isolated from the soil of Beishan, Xining City, Qinghai Province, and was classified as the genus of Pseudomonas sp. Its carboxymethyl cellulase (CMC) activity, exo-β-glucanases activity, and filter paper activity (FPA) were 25.35, 22.33, 20.58 U·mL ^-1, respectively. The strain was made into a liquid microbial inoculum and applied in a composting test at the rate of 10 mL·kg^-1 as the experiment group. Compared with the control group without application of microbial inoculum, the stack of the experiment group heated up quickly and the high temperature lasted longer. At the end of composting, the pH value and electrical conductivity of the experiment group were within a reasonable range, the seed germination index of the experiment group was 90%, which met the maturity standard, the moisture content of the stack dropped to 30% below, and the contents of total nitrogen and organic matter were 1.39% and 49.16%, respectively. Therefore, the isolated strain could be used in the rape straw composting, which could both accelerate the rate and improve the quality of composting in Qinghai.