辽宁省不同地区水稻二化螟对阿维菌素的抗药性研究

为明确辽宁省不同地区水稻二化螟对阿维菌素的抗药性变化以及抗性机制，连续3年采用毛细管点滴法和分光光度法测定水稻二化螟对阿维菌素的抗药性水平、羧酸酯酶（CarE）活性、多功能氧化酶（MFO）活性以及谷胱甘肽-S-转移酶（GST）活性。结果表明，辽宁省各地区水稻二化螟对阿维菌素的抗药性逐年上升，但仍处于低抗及敏感水平；CarE活性在不同地区的表现没有显著差异，MFO及GST活性均伴随着水稻二化螟对阿维菌素的抗药性上升呈现逐年增强的趋势，且部分地区间存在显著差异。由此可见，辽宁省各个地区水稻二化螟对阿维菌素的抗药性仍处于敏感水平，施用阿维菌素仍能取得较好的防治效果；水稻二化螟GST和MFO的活性变化能够较好的反映其对阿维菌素抗药性水平的变化。

Study on the Resistance of Rice Chilo suppressalis to Abamectin in Different Areas of Liaoning Province

The resistance level, carboxylesterase activity, multifunctional oxidase activity and glutathione S-transferase activity of rice Chilo suppressalis to abamectin were measured by topical application method and spectrophotometric method for three consecutive years, to clarify the changes of resistance to abamectin and the resistance mechanism of rice Chilo suppressalis in different areas of Liaoning Province. The resistance of Chilo suppressalis to abamectin in various regions of Liaoning Province was increasing year by year, but was still at low resistance and sensitivity levels. Carboxylesterase activity did not show significant differences in different regions, while multifunctional oxidase and glutathione S-transferase activities showed a trend of increasing year by year along with the resistance of Chilo suppressalis to abamectin, and there were significant differences in some regions. The resistance of Chilo suppressalis to abamectin was still at a sensitive level in various regions of Liaoning Province, and the application of abamectin could still achieve good control effects. The changes of glutathione S-transferase and multifunctional oxidase activities of Chilo suppressalis could better reflect the changes of its resistance level to abamectin.