| 脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性 |
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| 引用本文： | 闫梅,姚彦东,牟开萍,淡媛媛,李伟泰,廖伟彪.脱落酸通过提高抗氧化酶活性与基因表达参与富氢水增强番茄幼苗抗旱性[J].浙江农业学报,2022,34(9):1901-1910. |
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| 作者姓名： | 闫梅 姚彦东 牟开萍 淡媛媛 李伟泰 廖伟彪 |
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| 作者单位： | 甘肃农业大学 园艺学院,甘肃 兰州 730070 |
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| 基金项目： | 国家重点研发计划(2018YFD1000800);国家自然科学基金(32072559);国家自然科学基金(31860568);国家自然科学基金(31560563);国家自然科学基金(31160398);中国博士后科学基金(20100470887);中国博士后科学基金(2012T50828);甘肃省自然科学基金(1606RJZA073);甘肃省自然科学基金(1606RJZA077);甘肃省教育厅创新创业团队项目(2018C-14) |
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| 摘 要： | 以15%聚乙二醇6000(PEG-6000)处理模拟干旱胁迫，研究了氢气(H2)和脱落酸(ABA)在番茄(Solanum lycopersicum L.)幼苗抵抗干旱胁迫中的作用及其相互关系。结果表明，外源H2和ABA均可促进番茄在干旱胁迫下的生长，其中富氢水(HRW,H2供体)和ABA最适浓度分别为75%和150μmol·L-1。干旱胁迫下，HRW处理能显著增加番茄幼苗叶片内源ABA含量及其关键基因表达。同时，HRW和ABA单独及其共同处理的番茄幼苗叶片丙二醛(MDA)含量较PEG处理分别降低了34.8%、32.6%和37.0%;而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性及其基因表达显著高于PEG处理。另外，ABA抑制剂氟啶酮(FLU)抑制了HRW对干旱胁迫下番茄幼苗生长的促进作用，说明H2促进干旱胁迫下番茄幼苗的生长可能需要ABA的参与。FLU抑制了HRW对干旱胁迫下叶片MDA含量的降低作用。此外，FLU抑制了HRW对干旱胁迫下叶片...
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| 关 键 词： | 干旱 富氢水 番茄 丙二醛含量 抗氧化酶活性 基因表达 |
| 收稿时间： | 2021-03-24 |
Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings |
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| YAN Mei,YAO Yandong,MOU Kaiping,DAN Yuanyuan,LI Weitai,LIAO Weibiao.Involvement of abscisic acid in hydrogen gas-enhanced drought resistance by improving antioxidant enzyme activity and gene expression in tomato seedlings[J].Acta Agriculturae Zhejiangensis,2022,34(9):1901-1910. |
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| Authors: | YAN Mei YAO Yandong MOU Kaiping DAN Yuanyuan LI Weitai LIAO Weibiao |
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| Institution: | College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The 15% macrogol 6000 (PEG-6000) was used to investigate the effect of hydrogen gas (H2) and abscisic acid (ABA) on drought resistance of tomato (Solanum lycopersicum L.) seedlings and their interaction. The results showed that both H2 and ABA promoted the growth of tomato seedlings under drought stress, and the optimal concentrations of HRW and ABA were 75% and 150 μmol·L-1, respectively. HRW treatment could significantly increase endogenous ABA content and the related gene expression in tomato seedling leaves under drought stress. Meanwhile, HRW and ABA alone or together significantly decreased malondialdehyde (MDA) content by 34.8%, 32.6% and 37.0%, whereas HRW and ABA significantly increased the superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity and their gene expression in comparison with PEG treatment. Besides, fluridone (FLU), a synthetic inhibitor of ABA, significantly diminished the positive effect of HRW on tomato seedlings growth, showing that ABA was required for H2-promoted growth of tomato seedlings under drought stress. FLU suppressed the roles of HRW in MDA content of tomato leaves under drought stress. In addition, FLU suppressed the promotive roles of HRW in the activity of SOD, CAT and APX and their gene expression, indicating that ABA was involved in H2-enhanced the antioxidant enzyme activities and the realted gene expression under drought stress. Together,the resultsed showed that ABA might be involved in H2-enhanced the drought resistance of tomato seedlings by decreasing the content of MDA and increasing antioxidant enzyme activities and their gene expression. |
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| Keywords: | drought stress hydrogen-rich water tomato malondialdehyde content antioxidant enzyme activity gene expression |
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