木耳菌糠培养球孢白僵菌的条件筛选及优化

白僵菌是一种虫生真菌，目前在农林害虫防治中取得了明显的效果。为了进一步促进白僵菌农药的发展，开发低成本的白僵菌发酵方法或优化其发酵条件是不可缺少的一环。本文尝试以木耳菌糠作为主要培养基液体发酵球孢白僵菌，通过单因素试验和正交试验确定菌糠培养基配方并优化发酵条件。结果表明，废弃菌糠适宜作为球孢白僵菌的主要培养基，当料液比为1:4，补充0.3%硫酸锰、0.5%硝酸铵，接菌量10%，培养温度为25 ℃，培养时间为4 d时，球孢白僵菌的产孢量达到了2.0×10⁹个孢子/mL。研究证实了木耳菌糠培养球孢白僵菌的可行性，希望为低成本发酵其他生防菌提供新思路。

Screening and Optimization of Conditions for Cultivating Beauveria bassiana with Spent Fungus Substrates

Beauveria bassiana is an entomogenous fungus showing significant control of agricultural and forestry insect pests.To further promote the development of B.bassiana pesticide,it is indispensable to develop low-cost B.bassiana fermentation methods or optimize its fermentation conditions.Using spent mushroom substrates as the main medium to cultivate B.bassiana,this study preliminarily optimized the composition of the culture medium and the conditions for cultivating B.bassiana by single-factor experiments and orthogonal experiments.The results showed that spent mushroom substrates were suitable as the main culture medium for B.bassiana.When the material-to-liquid ratio is 1:4,with addition of 0.3% manganese sulfate,0.5% ammonium nitrate,and 10% inoculation amount,at a cultivating temperature of 25℃ and a cultivating period of 4 d,the spore production of B.bassiana reached 2.0×10⁹ conidia/mL.The results confirm the feasibility of cultivating B.bassiana with spent mushroom substrates,which may shed light on low-cost fermentation of other biocontrol fungi.