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解淀粉芽胞杆菌TR2对草莓土壤酶活性的影响与防病促生作用
引用本文:赵雨萌,缪佩佩,王旭东,高坦坦,史晓艺,贾绍雷,任争光,赵晓燕. 解淀粉芽胞杆菌TR2对草莓土壤酶活性的影响与防病促生作用[J]. 中国生物防治学报, 2022, 38(2): 495-501. DOI: 10.16409/j.cnki.2095-039x.2022.02.014
作者姓名:赵雨萌  缪佩佩  王旭东  高坦坦  史晓艺  贾绍雷  任争光  赵晓燕
作者单位:北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室,北京 102206
基金项目:北京市教委科技面上项目(KM201910020009);北京市农林科学院植物保护环境保护研究所技术服务合同(201900644)
摘    要:为探究生防菌防病促生的土壤微生态作用机制,本试验以生防解淀粉芽胞杆菌TR2对草莓进行灌根处理,利用比色法和滴定法对不同时间段的土壤酶活性进行测定和比较,并调查了草莓生长相关数据和草莓根腐病的发生率。结果表明:解淀粉芽胞杆菌TR2处理后,草莓根际土壤脲酶、蔗糖酶、过氧化氢酶和磷酸酶的活性明显高于对照,且持续时间长,到第60 d仍有明显促进作用。TR2处理对草莓根际土壤脲酶活性和蔗糖酶活性的促进作用较好,分别从第7 d和第15 d开始表现明显促进作用,第30 d时最好,脲酶活性比对照高出215%,蔗糖酶活性比对照高出124%。TR2处理从第15 d和第30 d开始分别表现对磷酸酶活性和过氧化氢酶活性的明显促进作用;对过氧化氢酶活性的促进作用在第60 d时最好,比对照高出43%;对磷酸酶活性的促进作用在第15 d时最好,酸性磷酸酶和碱性磷酸酶活性比对照分别增加69%和45%。解淀粉芽胞杆菌TR2处理后草莓的根长、根重、叶面积和最大单果重等各项生长指标都优于对照组,对根长的促进作用在20%左右,根重在第60 d时比对照组高出92%,15 d时平均最大叶面积比对照大10 cm2,第30 d时平均每株比对照多长出1.2个新叶。解淀粉芽胞杆菌TR2处理有利于降低根腐病的发病率,防效在25.0%左右。

关 键 词:草莓  解淀粉芽胞杆菌  土壤酶活  促生作用  防病作用  
收稿时间:2021-08-05

Influences of Bacillus amyloliquefaciens TR2 on Soil Enzyme Activities and Its Effects on Disease Control and Growth Promotion in Strawberry
ZHAO Yumeng,MIAO Peipei,WANG Xudong,GAO Tantan,SHI Xiaoyi,JIA Shaolei,REN Zhengguang,ZHAO Xiaoyan. Influences of Bacillus amyloliquefaciens TR2 on Soil Enzyme Activities and Its Effects on Disease Control and Growth Promotion in Strawberry[J]. Chinese Journal of Biological Control, 2022, 38(2): 495-501. DOI: 10.16409/j.cnki.2095-039x.2022.02.014
Authors:ZHAO Yumeng  MIAO Peipei  WANG Xudong  GAO Tantan  SHI Xiaoyi  JIA Shaolei  REN Zhengguang  ZHAO Xiaoyan
Affiliation:Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture Rural Affairs/College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China
Abstract:In order to explore the soil microecological mechanisms of biocontrol bacteria in disease prevention and growth promotion, the strawberry plants were inoculated with Bacillus amyloliquefaciens TR2 through root-drenching method in this study. The soil enzyme activities in different periods were analyzed by use of colorimetry and titration, and the strawberry growth and the incidence of strawberry root rot were investigated. The results showed that the activities of urease, sucrase, catalase and phosphatase in the rhizosphere soil of the strawberry plants were significantly higher than those in the control group after inoculating with B. amyloliquefaciens TR2, and the promotion lasted for a long time until the 60th day. The urease and sucrase activities were increased in the strawberry rhizosphere soil treated with B. amyloliquefaciens TR2, and the significant promoting effect were detected from the 7th and 15th day respectively, and reached the maximum on the 30th day, as that the activity of urease and sucrase was 215% and 124% higher than that of the control, respectively. The phosphatase and catalase activities were also dramatically enhanced by inoculation of B. amyloliquefaciens TR2 from the 15th day and 30th day, respectively. The increase of the catalase activity reached a peak on the 60th day, which was 43% higher than that of the control. While, enhancement of the phosphatase activity was the highest on the 15th day as that the activity of acid phosphatase and alkaline phosphatase increased by 69% and 45%, respectively, compared with that in the control group. Growth of the strawberry plants were improved by treating with B. amyloliquefaciens TR2, as that root length, root weight, leaf area and maximum of single fruit weight were better than those in the control group. B. amyloliquefaciens TR2 increased the root length by about 20% and root weight by 92% compared with that in the control group on the 60th day and the average maximum leaf area was 10 cm2 bigger than that in the control group on the 15th day. Besides, each plant produced 1.2 more leaves than that of the control on the 30th day. The incidence of root rot in the experimental group treated with B. amyloliquefaciens TR2 was reduced by about 25.0%.
Keywords:strawberry  Bacillus amyloliquefaciens  soil enzyme activity  growth promotion  disease prevention  
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