武夷菌素对活体微生物农药的影响

本文以武夷菌素为主要对象，以牛津杯抑菌测试法和菌丝生长抑制法观察武夷菌素对几种常见的真菌类活体微生物农药之间的影响。武夷菌素对于真菌孢子的抑制试验结果显示，武夷菌素在高浓度处理下对球孢白僵菌的抑制作用显著大于金龟子绿僵菌，其中对球孢白僵菌的最小抑菌浓度为400 mg/L，对金龟子绿僵菌的最小抑菌浓度为200 mg/L。武夷菌素对绿色木霉、哈茨木霉、淡紫拟青霉菌丝生长率的抑制试验结果显示：武夷菌素100 mg/L浓度对于三种真菌菌丝生长的抑制率分别为64.6%、48.6%、39.2%，进行毒力回归方程计算，数据表明武夷菌素对绿色木霉的EC₅₀为57.13 mg/L，对哈茨木霉的EC₅₀为109.81 mg/L，对淡紫拟青霉的EC₅₀为170.03 mg/L。武夷菌素在防治病害过程中实际用药量约100 mg/L，会对金龟子绿僵菌、球孢白僵菌、绿色木霉、哈茨木霉和淡紫拟青霉产生不同程度的抑制，建议应错开使用。本研究所获得的结果对指导武夷菌素及活体微生物农药的生物防治应用具有重要的意义，为生物防治技术集成提供理论基础。

Influence of Wuyiencin on Microbial Pestiside

This article takes Wuyiencin as the main object, and observes the effect of Wuyiencin on several common fungal live microbial pesticides by the Oxford Cup antibacterial test method and the mycelial growth inhibition method. The inhibition test results of Wuyiencin on fungal spores showed that the inhibitory effect of Wuyiencin on Beauveria bassiana was significantly higher than Metarhizium anisopliae under high concentration treatment.The minimum inhibitory concentration of B. bassiana was 400 mg/L, and the minimum inhibitory concentration of Metarhizium anisopliae was 200 mg/L. The inhibition rate of Wuyiencin on the mycelial growth rate of Trichoderma viride, Trichoderma harzianum, and Paecilomyces lilacinus showed that the inhibition rate of Wuyiencin 100 mg/L on the mycelial growth rate of the three fungi was 64.6%, 48.6%, 39.2%, respectively. Calculated by the virulence regression equation, the results showed that the EC₅₀ of Wuyiencin to Trichoderma viride is 57.13 mg/L, the EC₅₀ of T. harzianum was 109.81 mg/L, and the EC₅₀ of P. lilacinus was 170.03 mg/L. The actual dosage of Wuyiencin in the process of disease prevention and control was about 100 mg/L, which can inhibit M. anisopliae, B. bassiana, T. viride, T. harzianum and Paecilomyces lilacinus to varying degrees. It was recommended to stagger use. The results obtained in this study are of great significance for guiding the biological control application of Wuyiencin and live microbial pesticides, and provide a theoretical basis for the integration of biological control technology.