益生菌牙膏缓解犬牙菌斑和牙结石的研究

目的] 评价某公司生产的一款宠物用益生菌牙膏对犬牙菌斑和牙结石形成的缓解功效。方法] 选取口腔健康、体况良好的成年比格犬10只,配对设计随机分为阴性对照组（n=5）和益生菌牙膏组（n=5）;阴性对照组仅饲喂犬粮,益生菌牙膏组每次餐后给予约4 g/只益生菌牙膏,每天2次,连续使用28 d;试验第0天对所有试验犬牙齿进行洁牙抛光处理,试验第14天和第29天分别对比格犬牙菌斑进行评分,第29天对牙结石进行评分并检测口腔气体挥发性化合物（H₂S+CH₃SH）浓度;当牙菌斑和牙结石评分均值以及口腔气体挥发性化合物浓度均值减少幅度≥15%时,定义为益生菌牙膏效果显著。结果] 与阴性对照组相比,益生菌牙膏组在给予益生菌牙膏第14天时,牙菌斑评分均值减少幅度为-0.36%（P>0.05）,第29天牙菌斑评分均值减少幅度为21.47%（P<0.05）;第29天益生菌牙膏组牙结石评分均值减少幅度为6.54%（P>0.05）;第29天益生菌牙膏组犬口腔气体挥发性化合物浓度均值减少幅度为-44.59% （P>0.05）。结论] 在试验期内,该款益生菌牙膏能够有效抑制犬牙菌斑的形成,但不具有减少牙结石形成和降低口腔气体挥发性化合物浓度的功效。

Efficacy Evaluation of a Probiotic Toothpaste in Relieving Canine Dental Plaque and Calculus

Objective] This study was conducted to evaluate the efficacy of a probiotic toothpaste for pet use produced by a company in relieving the formation of dental plaque and calculus in dogs. Method] Ten adult Beagle dogs with healthy oral cavity and good physical condition were randomly divided into a negative control group （n=5） and a probiotic toothpaste group （n=5）. The negative control group was fed with dog food alone, and the probiotic toothpaste group was given about 4 g probiotic toothpaste per dog twice a day for consecutive 28 days after each meal. The teeth of all the experimental dogs were cleaned and polished on day 0 of experiment. The dental plaque were scored on day 14 and day 29 of experiment, respectively. The dental calculus and the concentrations of volatile compounds （H₂S+CH₃SH） in oral gas were scored and assessed on day 29 of experiment, respectively. Probiotic toothpaste was defined as having a significant efficacy when the mean values of scores regarding dental plaque and calculus formation as well as those of concentrations of volatile compounds in oral gas were reduced by 15% or higher. Result] Compared with the control group, the mean values of scores regarding dental plaque formation of the probiotic toothpaste group were decreased by -0.36% （P>0.05） and 21.47% （P<0.05） on day 14 and day 29 of experiment, respectively; the mean values of score regarding dental calculus formation and concentrations of volatile compounds in oral gas of the probiotic toothpaste group were dropped by 6.54% （P>0.05） and -44.59% （P>0.05） on day 29 of experiment, respectively. Conclusion] Within the experimental period, the tested probiotic toothpaste effectively inhibited the formation of dental plaque in Beagle dogs, but had no efficacy on relieving the formation of dental calculus and reducing the concentrations of volatile compounds in oral gas.