氯通道抑制剂缓解栽培大豆盐伤害的离子特征

为了从离子响应的视角探究氯通道抑制剂缓解栽培大豆幼苗盐伤害的作用机理,以大豆栽培品种绥农35为试验材料,采用大豆幼苗期盐胁迫外加氯通道抑制剂的方法,比较不同处理幼苗的相关生理指标、解剖结构和离子组的差异。结果显示:外加不同氯离子抑制剂对盐胁迫作用效果不同,分别表现为ZnCl₂能缓解栽培大豆幼苗的盐伤害作用,但尼氟灭酸(NFA)和蒽-9-羧酸(9-AC)则相反,加重了盐害作用;外加Zn²⁺,根部次生导管孔径回升,皮层细胞厚度减少,有利于降低根部总消耗,缩短水分和矿质离子横向运输的距离;外加Zn²⁺,叶部叶绿素含量、叶绿素荧光参数PSⅡ最大光化学效率(F_v/F_m)值,N {\mathrm{O}}_3^{-}以及微量元素(Fe, Mn, Cu和Zn)含量均回升,而Cl^-含量回落介于对照组与盐处理组之间。综上表明,盐胁迫下外加Zn²⁺可重建幼苗叶部的离子稳态,降低叶部Cl^-毒害,提高幼苗抗氧化能力和光合能力,有效缓解大豆幼苗受到的伤害。

Ion characteristics on chloride channel blocker ameliorating salt injury to Glycine max

To elucidate the mechanism on chloride channel inhibitor Z {{\mathrm{n}}^2}^{+}in ameliorating salt injury to soybean from the perspective of ion response, in this study, Glycine max cultivar Suinong 35 was used as the experimental material. The differences in physiological indexes, anatomical structure and ionomics of seedlings under different treatments were compared by the method of NaCl stress plus chloride channel inhibitors. The results showed that under NaCl stress plus additional three chloride channel inhibitors, ZnCl₂ could ameliorate salt injury in soybean to a certain extent, but nifluoric acid (NFA) and anthracene-9-carboxylic acid (9-AC) showed the reverse. Under NaCl stress plus Zn²⁺, the diameter of secondary vessel in root was markedly recovered, while cortex thickness in roots was decreased, which was helpful to reduce the metabolic cost and shorten the distance of water absorption from root to vascular cylinder. Under NaCl stress plus Zn²⁺, total chlorophyll content, F_v/F_m, N {{\mathrm{O}}_3}^{-}and microelement contents (Fe, Mn, Cu and Zn) rose again, while leaf Cl^- content was reduced between the control and NaCl-stressed seedlings. In summary, NaCl stress plus Zn²⁺ can effectively ameliorate salt injury to soybean seedlings by reestablishing ion homeostasis, which reduces Cl^- toxicity in leaves and improves antioxidant capacity and photosynthetic capacity.