基于血浆非靶代谢组学探究急性笼养应激对蛋鸭代谢的影响

随着环保政策改革和养殖业的集约化、规模化发展，蛋鸭叠层式笼养模式的应用越来越广泛，笼养应激对蛋鸭的影响逐渐受到重视。本研究旨在利用非靶向代谢组学分析蛋鸭平养与笼养模式下血浆代谢物的差异。随机挑选平养组(floor-water rearing system, FWR组)和叠层式笼养组(cage-rearing system, CR组)蛋鸭各10只，跖骨静脉采血，通过高效液相色谱-质谱(HPLC-HRMS)非靶向代谢组学技术获得20个在不同饲养模式下的血浆代谢物图谱，经T检验等分析，取差异倍数(Fold-change)ratio≥2或ratio≤1/2、变量重要性(variable importance in projection, VIP)>1、q值<0.05为筛选条件得到显著差异代谢物并作通路富集分析。结果共筛选到差异代谢物30种，其中上调表达的差异代谢物14种(P<0.05),下调表达的差异代谢物16种(P<0.05)。差异代谢物种类主要有甘油磷脂(glycerophospholipids, 16种)、脂肪酰基(fatty acyls, 4种)、羧酸及其衍生物...

Effect of Acute Cage Stress on the Metabolism of Laying Ducks Studied by Plasma Nontargeted Metabolomics

Investigating caging stress in laying ducks has become extremely important since the environmental policies have changed and the duck industry has grown. This study aims to explore the plasma metabolite profiles of laying duck raised in floor-water rearing system (FWR) and cage-rearing system (CR) using nontargeted metabolomics. The metatarsal vein blood was taken from ten laying ducks from each experimental group for HPLC-HRMS. Differential metabolites were identified with Fold-change ratio≥2 or ≤1/2, VIP>1 and q value<0.05. A total of 30 differential metabolites were obtained based on the screening conditions, of which 14 were up-regulated (P<0.05) and 16 were down-regulated (P<0.05). Differential metabolites included 16 glycerophospholipids, 4 fatty acyls, 2 carboxylic acids and derivatives, as well as phenylpropionic acid, phenols, organic sulfuric acids and derivatives, sterol lipids, purine nucleosides, organooxygen compounds, steroids and steroid derivatives, steroids and steroid derivatives. Primary bile acid biosynthesis and glycerophospholipid metabolism (P<0.01), as well as the secondary bile acid biosynthesis and taurine and hypotaurine metabolism (P<0.05) were found to be enriched by the pathway enrichment analysis. The results showed substantial variations in plasma bile acids, taurine, and glycerophospholipids between FWR and CR. The insights gained from this study may be useful for the development and promotion of waterfowl husbandry by clarifying the mechanism of caging stress in laying duck industry.