木霉共培养发酵对黄瓜枯萎病的防治效果

为明确深绿木霉HB20111和哈茨木霉TW21990共培养发酵对黄瓜枯萎病的防治作用,采用对峙培养法观察两菌株的亲和性,通过浸种抑菌萌发试验筛选共培养发酵的最佳条件,并在温室条件下验证其生防效果.结果表明:在PDA平板上25℃对峙培养5 d,两株木霉亲和性较好.两菌株等量接种发酵培养基,25℃、130 r/min振荡培...

Control Effect of Co-cultured Fermentation of Two Trichoderma Strains on Cucumber Fusarium Wilt

To ascertain the potential use of co-cultured two Trichoderma strains (T. atroviride HB20111 and T. harzianum TW21990) to control Fusarium oxysporum f. sp. cucumerium Owen (FOC), the optimum condition for co-cultured fermentation were detected by plate confrontation culture and seeds germination test, and the control efficiency were detected by pot test in greenhouse. The results showed that HB20111 and TW21990 had good compatibility after co-cultivation on PDA plate at 25℃ for 5 days. A co-culture with the inoculation of the two organisms in the ratio of 1:1, shaked in 130 r/min for 72 h at 25℃, the co-cultured fermentation diluted 500 times could enhance root lengths of cucumber seeds (P<0.05) by soaking seeds treatment. Greenhouse test demonstrated that co-cultured fermentation diluted 500 times had the control efficacy of 73.65% and increased the seedling fresh weight at rate of 35.64% by soaking seeds treatment, respectively. Compared with singly-cultured strain HB20111 and TW21990, the efficiencies of the paired were significantly increased by 6.76%, 9.46% and 11.54%, 13.31%, respectively. Co-cultured fermentation treatment was also significantly reduced the FOC DNA copy numbers in cucumber rhizosphere soils (P<0.05). Therefore, co-cultured HB20111 and TW21990 had significant synergistic effect on the control of cucumber Fusarium wilt, which provided a new insight into development of highly efficient biocontrol agents.