| 北五味子组织培养研究 |
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| 引用本文： | 张晓薇,夏媛,李凤兰,胡宝忠.北五味子组织培养研究[J].安徽农业科学,2009,37(9):3933-3935. |
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| 作者姓名： | 张晓薇 夏媛 李凤兰 胡宝忠 |
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| 作者单位： | 东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030;东北农业大学生命科学学院,黑龙江哈尔滨,150030 |
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| 摘 要： | 目的]研究北五味子的组织培养,为其快速繁殖提供参考。方法]以北五味子的嫩茎段、叶片、叶柄为外殖体,MS和改良B5为基本培养基,使用不同激素不同浓度配比,优选不同外殖体诱导愈伤组织的最佳培养基。结果]嫩茎段的最佳诱导培养基为：改良B5＋6-BA 2.5 mg/L＋NAA 0.1 mg/L 叶片的最佳诱导培养基为：改良B5＋6-BA 2.0 mg/L＋NAA 0.1 mg/L＋2,4-D 1.0 mg/L 叶柄的最佳诱导培养基为：改良B5＋6-BA 2.5 mg/L＋2,4-D 1.0 mg/L。7种培养基均未诱导出根。结论]改良B5培养基优于MS培养基,嫩茎段为愈伤组织诱导的最佳外殖体。
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| 关 键 词： | 北五味子 组织培养 植物生长调节剂 |
Study on the Tissue Culture of Schisandra chinensis (Turcz.) Baill. |
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| ZHANG Xiao-wei et al.Study on the Tissue Culture of Schisandra chinensis (Turcz.) Baill.[J].Journal of Anhui Agricultural Sciences,2009,37(9):3933-3935. |
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| Authors: | ZHANG Xiao-wei |
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| Institution: | ZHANG Xiao-wei et al(School of Life Sciences,Northeast Agriculture University,Harbin,Heilongjiang 150030) |
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| Abstract: | Objective]The aim was to study the tissue culture of Schisandra chinensis and provide preference for papid propagation.Method]The tender stem segments,leaves and petioles of Schisandra chinensis were cultured as explants with various concention media(MS and improvement B5) and different kinds of plant growth regulator.Result]The result showed that improvement B5 medium supplemented with 2.5 mg/L 6-BA and 0.1 mg/L NAA had the best effect on inducement of the tender stem segments,improvement B5 medium supp... |
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| Keywords: | Schisandra chinensis Tissue culture Plant growth regulator |
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