| 单细胞凝胶电泳检测家蚕冷冻精子的DNA损伤 |
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| 引用本文： | 陈田飞,吴大洋. 单细胞凝胶电泳检测家蚕冷冻精子的DNA损伤[J]. 蚕业科学, 2005, 31(3): 366-369 |
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| 作者姓名： | 陈田飞 吴大洋 |
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| 作者单位： | 西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716;西南农业大学蚕学与生物技术学院,农业部蚕桑学重点开放实验室,重庆,400716 |
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| 基金项目： | 西南农业大学校科研和教改项目 |
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| 摘 要： | 采用单细胞凝胶电泳法检测家蚕冷冻精子DNA损伤,结果未加冷冻保护剂(含10%DMSO的Grace s me-dium)的部分精子SCGE图呈明显的彗星状,其DNA损伤程度达56%,与对照组相比差异显著,表明DNA受到了一定程度的损伤;而添加冷冻保护剂的精液无论在-80℃下保存4个月还是在液氮中保存1年,其精子的SCGE图仍然呈圆形,DNA损伤程度与对照无显著性差异。
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| 关 键 词： | 家蚕 精子 单细胞凝胶电泳 DNA 损伤 |
| 文章编号： | 0257-4799(2005)03-0366-04 |
| 修稿时间： | 2004-12-22 |
Detection of DNA Damage of Silkworm Cryopreservation Sperm Using Single Cell Gel Electrophoresis |
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| Chen Tianfei,Wu Dayang. Detection of DNA Damage of Silkworm Cryopreservation Sperm Using Single Cell Gel Electrophoresis[J]. Acta Sericologica Sinica, 2005, 31(3): 366-369 |
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| Authors: | Chen Tianfei Wu Dayang |
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| Abstract: | In this paper, we inspected DNA damage level of silkworm cryopreservation sperm using the single cell gel electrophoresis assay (SCGE) method. The result showed that the SCGE pattern of sperm preserved without cryoprotectant presents apparent comet-shape and the DNA damage is obvious, which indicated that DNA suffer a certain degree damages; whereas the SCGE pattern of sperm, which has been preserved with cryoprotectant at -80 ℃ or in liquid nitrogen, remained circular, and there is no significant difference of DNA damage compared to the control. |
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| Keywords: | Bombyx mori Sperm SCGE DNA Damage |
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