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Distribution of the Consensus Sequences of ABC Transporter Gene among Several Taxonomically Distinct Phytopathogenic Fungi
Authors:Young-Jin LEE  Hiroshi HAMAMOTO  Ryoji NAKAUNE  Osamu NAWATA  Yoshiyuki MAKIZUMI  Katsumi AKUTSU  Tadaaki HIBI
Affiliation:(1) Department of Agricultural and Environmental Biology, The University of Tokyo, Bunkyo-ku, Tokyo 113–8657, Japan, JP;(2) Bio-oriented Technology Research Advancement Institution (Brain), Nisshin, Ohmiya 331–0044, Japan, JP;(3) Faculty of Agriculture, Ibaraki University, Ami-machi, Ibaraki 300–0393, Japan, JP
Abstract:The distribution of ABC (ATP-binding cassette) transporter genes among several taxonomically distinct phytopathogenic fungi was investigated by Southern blot hybridization, polymerase chain reaction assays and partial sequencing. Consensus sequences of the ABC transporter gene, which might be concerned with multidrug resistance against fungicides and with pathogenicity in phytopathogenic fungi, were found in all of the examined phytopathogenic fungi, which belonged to the Mastigomycotina, Ascomycotina, Basidiomycotina and Deuteromycotina. Received 24 August 2000/ Accepted in revised form 5 December 2000
Keywords:: ABC transporter   phytopathogenic fungi   PCR.
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