Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest,Increased Cell Size and Apoptosis in Saccharomyces cerevisiae |
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| Authors: | Juan A. Rubiolo Eva Ternon Henar López-Alonso Olivier P. Thomas Félix V. Vega Mercedes R. Vieytes Luis M. Botana |
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| Affiliation: | 1.Department of Pharmacology, Faculty of Veterinary, University of Santiago de Compostela (USC), Campus Lugo, Lugo 27002, Spain; E-Mails: (J.A.R.); ;2.Department of Physiology, Faculty of Veterinary, University of Santiago de Compostela (USC), Campus Lugo, Lugo 27002, Spain; E-Mails: (F.V.V.); (M.R.V.);3.Nice Institute of Chemistry-PCRE, UMR 7272 CNRS, Faculty of Sciences, University of Nice Sophia Antipolis, Parc Valrose 06108 Nice, France; E-Mails: (E.T.); (O.P.T.) |
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| Abstract: | In this paper, we show the effect of crambescidin-816, -800, and -830 on Saccharomyces cerevisiae viability. We determined that, of the three molecules tested, crambescidin-816 was the most potent. Based on this result, we continued by determining the effect of crambescidin-816 on the cell cycle of this yeast. The compound induced cell cycle arrest in G2/M followed by an increase in cell DNA content and size. When the type of cell death was analyzed, we observed that crambescidin-816 induced apoptosis. The antifungal effect indicates that crambescidins, and mostly crambescidin-816, could serve as a lead compound to fight fungal infections. |
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| Keywords: | crambescidine-816 antifungal cell cycle arrest apoptosis |
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