| 产木聚糖酶菌株的分离及木聚糖酶的固定化 |
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| 引用本文： | 王瑞君. 产木聚糖酶菌株的分离及木聚糖酶的固定化[J]. 湖北农业科学, 2012, 51(19): 4337-4339 |
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| 作者姓名： | 王瑞君 |
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| 作者单位： | 宜春学院江西省天然药物活性成分研究重点实验室/化学与生物工程学院,江西宜春,336000 |
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| 基金项目： | 江西省宜春市科技局资助项目(JXYC2010KNB003);江西省教育厅科研项目(JXJG-10-14-13) |
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| 摘 要： | 从草鱼肠道中分离筛选出6株产木聚糖酶的菌株,从中挑选产酶量最大的菌株测定酶液活力,并采用壳聚糖、海藻酸钠等固定游离酶.结果表明,与游离酶相比,壳聚糖固定酶的最适pH向碱性方向偏移,海藻酸钠固定酶的最适pH向酸性方向偏移.与游离酶相比,固定化酶有较高的最适温度和较强的热稳定性.
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| 关 键 词： | 木聚糖酶 菌株分离 固定化 |
Isolation of Xylanase Producing Strain and Immobilization of Xylanase |
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| WANG Rui-jun. Isolation of Xylanase Producing Strain and Immobilization of Xylanase[J]. Hubei Agricultural Sciences, 2012, 51(19): 4337-4339 |
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| Authors: | WANG Rui-jun |
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| Affiliation: | WANG Rui-jun(Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province/Department of Chemistry and Bioengineening,Yichun University,Yichun 336000,Jiangxi,China) |
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| Abstract: | 6 xylanase producing strains were screened from the intestinal tract of grass carp,among which the strain with the highest xylanase yield was adopted to detect the enzyme activity of xylanase.Furthermore,the xylanase was immobilized by chitosan and sodium alginate.The results showed that compared with free enzyme,the optimal pH for chitosan immobilized xylanase was higher while for sodium alginate immobilized xylanase was lower.The optimal temperature for the immobilized xylanase was higher than that of free enzyme.The immobilized xylanase also showed broader temperature adaptability. |
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| Keywords: | xylanase strain isolation immobilization |
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