Intranasal delivery of nanoparticles encapsulating BPI3V proteins induces an early humoral immune response in mice |
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| Institution: | 1. Agri Food Biosciences Institute, Veterinary Sciences Division, Stoney Road, Stormont, Belfast BT4 3SD, UK;2. Animal and Bioscience Research Department, Animal & Grassland Research and Innovation Centre, Teagasc, Grange, Dunsany, Co. Meath, Ireland;3. Veterinary Sciences Centre, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Ireland;1. Department of Small Animals, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, UFSM. Avenida Roraima n° 1000, Camobi, Santa Maria, RS, CEP 97105-900, Brazil;2. Department of of Animal Science, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, Chapecó, Santa Catarina, CEP 89815-630, Brazil;3. Department of Chemistry, Universidade Federal de Santa Maria, Avenida Roraima, Prédio 18 – Cidade Universitária; Faixa de Camobi, KM 9; Bairro Camobi; Santa Maria/RS, CEP 97105-900, Brazil;4. Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil;5. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Campus Universitário, Camobi – Santa Maria, Rio Grande do Sul, CEP 97105-000, Brazil;6. Department of Pathology, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, prédio 97B, UFSM – Avenida Roraima, n° 1000, Camobi, Santa Maria, Rio Grande do Sul, CEP 97105-900, Brazil;1. Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany;2. State Office for Agriculture, Food Safety and Fishery Mecklenburg-Western Pommerania (LALLF M-V), Thierfelderstraße 18, 18059 Rostock, Germany;3. Clinic for Ruminants and Swine, Department of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany;1. Virginia Commonwealth University, VCU Pauley Heart Center, Richmond, VA, USA;2. Victoria Johnson Research Laboratories, Richmond, VA, USA;3. University of Colorado, Department of Medicine, Aurora, CO, USA;1. School of Veterinary Medicine, University College Dublin (UCD), Dublin 4, Ireland;2. Central Veterinary Research Laboratory, Backweston, Celbridge, Co. Kildare, Ireland;3. Immune Solutions Ltd, Centre for Innovation, The University of Otago, Dunedin, New Zealand;4. Department of Bovine Tuberculosis, Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, UK |
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| Abstract: | Vaccine adjuvants are typically designed to stimulate both systemic and mucosal immune responses. Polymeric nanoparticles have been used as adjuvants in the development of vaccines against a number of viral pathogens and tested in laboratory animals. The objective of the study was to assess if synthetic bovine parainfluenza virus type-3 (BPI3V) peptide motifs and solubilised BPI3V proteins encapsulated in poly (dl-lactic-co-glycolide) (PLGA) nanoparticles (NPs) induce specific humoral immune responses in a mouse model following intranasal administration. BPI3V-specific and peptide specific IgG ELISAs were used to measure serum IgG levels to BPI3V. Intranasal delivery of PLGA nanoparticles encapsulating BPI3V proteins elicited an early, gradually increasing BPI3V-specific IgG response that persisted over the subsequent 6 weeks, suggesting slow, persistent release of antigen. PLGA-BPI3V particles administered intranasally induced a stronger IgG antibody response at an earlier time point compared with solubilised BPI3V antigen alone. Such an approach could be deployed in the development of new generation vaccines. |
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