In vitro increase of mean corpuscular volume difference (dMCV) as a marker for serum hypertonicity in dogs |
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| Institution: | 1. Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA;2. Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA;1. Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany;2. State Office for Agriculture, Food Safety and Fishery Mecklenburg-Western Pommerania (LALLF M-V), Thierfelderstraße 18, 18059 Rostock, Germany;3. Clinic for Ruminants and Swine, Department of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany;1. Unidad Regional Universitaria Sursureste, Universidad Autónoma Chapingo, Km 7.5, carretera Teapa-Vicente Guerrero, P.O. Box 29, CP 86800, Teapa, Tabasco, México;2. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Yucatán, Carr. Mérida-Xmatkuil km 15.5, CP 97100, Mérida, Yucatán, México;3. División Académica de Ciencias Agropecuarias, Universidad Juárez Autónoma de Tabasco, Carr. Villahermosa-Teapa, km 25, CP 86280, Villahermosa, Tabasco, México;1. University College of London, Institute of Orhopaedics and Musculoskeletal Science, Royal National Orthopaedics Hospital, Brockley Hill, HA4 7LP, Stanmore, United Kingdom;2. Royal Veterinary College, Hawkshead Lane, North Mimms, AL9 7TA, Hatfield, United Kingdom;3. Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Distrito de Rubião Junior, S/N. Caixa Postal 560, CEP 18618-970, Botucatu, São Paulo-SP, Brazil;4. Veterinary Teaching Hospital, University of Brasilia, L4 Norte, CEP 70910-900, Brasilia-DF, Brazil;1. Department of Small Animals, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, UFSM. Avenida Roraima n° 1000, Camobi, Santa Maria, RS, CEP 97105-900, Brazil;2. Department of of Animal Science, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, Chapecó, Santa Catarina, CEP 89815-630, Brazil;3. Department of Chemistry, Universidade Federal de Santa Maria, Avenida Roraima, Prédio 18 – Cidade Universitária; Faixa de Camobi, KM 9; Bairro Camobi; Santa Maria/RS, CEP 97105-900, Brazil;4. Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil;5. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Campus Universitário, Camobi – Santa Maria, Rio Grande do Sul, CEP 97105-000, Brazil;6. Department of Pathology, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, prédio 97B, UFSM – Avenida Roraima, n° 1000, Camobi, Santa Maria, Rio Grande do Sul, CEP 97105-900, Brazil |
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| Abstract: | Spurious increase in erythrocyte mean corpuscular volume (MCV) on automated cell analyzers is a well-characterized lab error in hypertonic patients. A difference between automated and manual MCV (dMCV) greater than 2 fl has been shown to predict hypertonicity in humans. The purpose of this study was to investigate dMCV as a marker for serum hypertonicity in dogs and to examine the relationship between dMCV and three methods of estimating serum tonicity: measured (OsMM), calculated (OsMC), and calculated effective (OsMCE) osmolalities. OsMC, OsMCE, and dMCV were calculated from routine blood values and OsMM was directly measured in 121 dogs. The dMCV of hypertonic dogs was significantly larger than that of normotonic dogs for all three osmolality methods. dMCV predicted hypertonicity as estimated by OsMM better than it predicted hypertonicity as estimated by OsMC and OsMCE. A cutoff of 2.96 fl yielded the best sensitivity (76%) and specificity (71%) for hypertonicity estimated by OsMM. |
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