| Analysis of Oleanolic Acid and Ursolic Acid,Potential Antifertility Agents in Moringa （Moringa oleifera） Seed |
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| 作者姓名： | Akwasi Ampofo-Yeboah Helet Lambrechts Danie Brink Fletcher Hiten Evans Afriyie-Gyawu |
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| 作者单位： | [1]Animal Sciences Department, Stellenbosch University, PMB X1, Stellenbosch 7602, South Africa [2]Fisheries ＆ Aquatic Resources Management Department, University for Development Studies, Tamale, Ghana [3]Genetics Department, Stellenbosch University, Stellenbosch 7602, South Africa [4]Central Analytical Facilities, Mass Spectrometry Unit, Stellenbosch University, Stellenbosch 7602, South Africa [5]Jiann-Ping Hsu College of Public Health, Georgia Southern University, Statesboro GA 30460-8015, USA |
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| 基金项目： | Acknowledgment The authors thank Division of Aquaculture, Stellenbosch University for funding this research to contribute to find solution for tilapia precocious breeding problem. |
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| 摘 要： | Oleanolic acid （OA） and ursolic acid （UA） are isomeric tritcrpenes that are difficult to separate chromatographically. Studies have indicated that both OA and UA have antifertility properties in several livestock species, suppressing attainment of puberty, gonad function and gamete production. Being able to determine the presence and quantity of the OA and UA in moringa and pawpaw seeds will allow for calculation of optimal inclusion levels of these acids in animal diets for antifertility-activity. The purpose of the study was thus to quantify the levels of OA and UA in Moringa and pawpaw seed powder by means of ultra-high performance liquid chromatography with electrospray ionization tandem mass spectrometry （UHPLC-ESI-MS/MS）. Only OA was detected in moringa seeds at a concentration level of 0.508 p.g/g ＋ 0.032 pg/g dry weight. UA and OA could not be detected in pawpaw seeds used in this study. The presence of OA in moringa seeds suggest that the seeds can be included in tilapia diets as a potential antifertility treatment to control precocious breeding in tilapia culture in small scale farming systems in Sub-Saharan Africa. Further studies are warranted to determine the levels of OA and UA composition in moringa seeds from other parts of the Sub-Saharan Africa （other than Zambia）.
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| 关 键 词： | 抗生育活性 齐墩果酸 熊果酸 种子 辣木 超高效液相色谱 浓度水平 电喷雾电离 |
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