‘白核’龙眼种子败育不同时期差异蛋白分析

 以‘白核’龙眼种子为试验材料, 比较两种蛋白质的提取方法, 确定了酚抽法提取龙眼种子蛋白。应用蛋白质组学研究技术, 分析了龙眼种子败育4个不同时期的蛋白质组变化, 共发现21个差异蛋白, 其中9个上调表达, 12个下调表达。通过MALD I/TOF /TOF2MS/MS质谱分析和蛋白质数据库检索, 共鉴定出12个差异蛋白, 分别为胞质苹果酸脱氢酶、丙酮酸脱氢酶、磷酸甘油酸变位酶、RuBisCO大亚基结合蛋白α、β亚基、17 kD HSP、抗坏血酸过氧化物酶、胞质抗坏血酸过氧化物酶、半胱氨酸蛋白酶、Desication-related protein以及两个未知蛋白。这些鉴定出的差异蛋白质与能量和物质代谢、分子伴侣功能、自由基清除和抗氧化作用、细胞凋亡等生理过程密切相关, 可能参与了龙眼种子败育过程。

Cloning and Expression Analysis of EG Genes in Litchi Fruit

Two diflferent full lengths of EG cDNAs,termed as LcEG1 and LcEG2,were isolated from litchi fruit using RT-PCR and RACE (rapid amplification of cDNA ends) methods. Sequence alignment showed that four typical Cys residues and two predicted glycosyl hydrolase active sites were observed in the deduced LcEGl and LeEG2 proteins.Northern blotting analysis showed that the level of LcEG1 mRNA accumulation graduallv decreased in pericarp but steadily increased in aril accompanying with fruit growth and development,while the expression of LcEG2 could only be detected in aril at early stage of fruit development.More importantlv.the accumulation of LcEG1 mRNA was higher in cracking-susceptible cuhivar Nuomici than that in cracking-resistant cuhivar Huaizhi at any stage of fruit development.Thus,it could be speculated that LcEG1 was closelv related to the growth and cracking of litchi fruit,while,LcEG2 was only related to the growth of fruit at the early phase.