体细胞核移植技术在转基因动物研究中的应用

利用基因工程技术将目的基因整合入动物体细胞染色体中，并将其作为供体核移植入受体－－去核卵母细胞构成重建胚，然后将其移植入假孕母体，待其妊娠、分娩，便可得到经定向遗传修饰的转基因克隆动物。本文就这一领域的发展历史、研究现状、应用前景及存在的问题等方面作一概述。

Applications of Somatic Cell Nuclear Transfer Technology in Transgenic Animal Research

Transgenic techmology has developed quickly in recent years.The gene of interest could be integrated into the chromosome of donor-somatic cell of animal.After donor is transferred into the recipient-enucleated oocyte,the reconstructed embryo is transplanted into the female animal to establish pregency until the birth of offspring.Thus,we can get atransgenic clone animal,which is genetically modified.This paper will review the history of development,the status of research,the future of applications and the existing problems in this field