Dietary Polyunsaturated Fatty Acid Supplementation Improves the Quality of Stallion Cryopreserved Semen |
| |
| Institution: | 1. Animal Science Department, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil;2. Animal Science Department, Federal University of Lavras, Lavras, Minas Gerais, Brazil;3. Veterinary Department, Federal University of Lavras, Lavras, Minas Gerais, Brazil;4. Animal Reproduction Department, College of Veterinary and Animal Science, University of Sao Paulo, São Paulo, SP, Brazil;1. Animal Reproduction, Department of Agrarian Sciences, University of the Azores, CITA-A, Angra do Heroísmo, Portugal;2. Animal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt;1. Animal Reproduction and AI Department, Veterinary Division, National Research Center, Dokki, Egypt;2. Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Egypt;3. Police Training Department, Ministry of Interior, Egypt;1. Department of Veterinary Medicine and Animal Production, Federico II University, Naples, Italy;2. Department of Political Science, Federico II University, Naples, Italy;3. Department of Biochemistry, Biophysics and General Pathology, II University of Naples, Naples, Italy;1. The Nutraceutical Alliance, 10526 4th Line, Campbellville, ON L0P 1B0, Canada;2. Freedom Health LLC, 65 Aurora Industrial Parkway, Aurora, OH 44202, USA;1. Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria;2. Department of Veterinary Physiology, Ahmadu Bello University, Zaria, Nigeria;1. Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, Davis, CA;2. Department of Animal Science, University of California, Davis, Davis, CA;3. Select Breeders Services, Inc., Chesapeake City, MD;4. Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, Davis, CA |
| |
| Abstract: | The objective was to assess the influence of polyunsaturated fatty acid supplementation on the quality of fresh, cooled, and frozen-thawed stallion semen. Ten stallions received their normal diet (control group) or normal diet plus 150 mL of polyunsaturated fatty acid (PUFA) linseed-based oil (PUFA group). Semen was collected every 15 days during 60 days. Stallions were reversed across the treatments after a sixty-day interval. Semen was evaluated at 2, 6, 12, and 24 hours after cooling and 24 hours after freezing. Motility (MOT), vigor, membrane viability, morphology, acrosome integrity, and osmotic tolerance test (OTT) were evaluated. In the frozen-thawed semen, sperm dynamic characteristics were analyzed by computer-assisted sperm analysis and thiobarbituric acid reactive substances (TBARs) determined. The effects of treatment, time, semen type, and their interactions were submitted to PROCMIX (SAS) and means compared by the Tukey test. There was no treatment effect on the quality of fresh and cooled semen. However, frozen-thawed semen MOT, vigor, and OTT were superior (P < .05) in control compared to PUFA group. An interactive effect of sample day by treatment was observed, such that, TBARs increased over time (P = .002) in the PUFA group after 15, 30, 45, and 60 days from the beginning of supplementation. Thus, sperm may become more susceptible to the reactive oxygen species, probably due to the incorporation of polyunsaturated fat in the cell membrane. The addition of PUFA-enriched oil may be an alternative for improving frozen-thawed semen quality by increasing its MOT and resistance to osmotic changes to which sperm cells are submitted during the freezing process. |
| |
| Keywords: | Cryopreservation Fertility Membrane integrity Spermatozoa motility Omega-3 |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
