Variation in TBX3 Gene Region in Dun Coat Color Polish Konik Horses |
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| Affiliation: | 1. Department of Horse Breeding, the University of Agriculture in Kraków, al. Mickiewicza 24/28, 30-059 Kraków, Poland;2. Department of Animal Genomics and Molecular Biology, National Research Institute of Animal Production, 1 Krakowska, 32-083 Balice, Poland;3. Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland;1. Departmento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias – Unesp, CEP 14.884-900, Jaboticabal, São Paulo, Brazil;2. Departmento Melhoramento e Nutrição Animal, Faculdade de Medicina Veterinária e Zootecnia - Unesp, Botucatu, São Paulo CEP 18.618-307, Brazil |
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| Abstract: | The Polish Konik is a primitive breed of horse, most likely descended directly from the wild extinct forest equus so-called Tarpan (Equus ferus ferus). The studbook of this breed is closed, and only individuals with blue dun in color with a well-developed black middorsal stripe and other primitive markings are entered. The aim of the presented study was the investigation of genetic background of dun phenotype in Polish Konik horses. A total of 93 Polish Koniks were investigated toward single nucleotide polymorphisms in a region of TBX3 gene by Sanger sequencing method. Obtained results confirmed that all investigated horses carry dun dilution genotype. Two new variants were identified which have not been previously described [WT/WT-G/G-A/G; WT/del-G/del-G/G]. Furthermore, our results showed in population of Polish Koniks occurrence of the rare genotype recently found in Estonian-native horses. |
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| Keywords: | Equus Genotyping Genome sequence Phenotype Sanger sequencing |
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