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Effect of Mineral Block Supplementation on In Vivo Digestibility and In Vitro Gas Production With Equine Fecal Bacteria
Institution:1. Chemokine Biology Research Laboratory (CBRL), Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de, México;2. Posgrado de Bioquímica, Universidad Nacional Autónoma de, México;3. Laboratorio de Pruebas Biológicas, Instituto de Química, Universidad Nacional Autónoma de, México;4. BGR Bundesansalt fur Geowissenschaften und Rohstoffe, Stilleweg 2, D-30655 Hannover, Germany;5. Departamento de Microscopia Electrónica, Instituto Mexicano del Petróleo, México;6. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana Unidad Cuajimalpa, México;7. Earth Science Division, Lawrence Berkeley National Laboratory, United States;1. School of Geoscience and Surveying Engineering, China University of Mining & Technology, Beijing 100083, PR China;2. State Key Laboratory of Coal Resources and Safe Mining, China University of Mining & Technology, Beijing 100083, PR China;3. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, 2 George Street, GPO Box 2434, Brisbane, Queensland 4001, Australia
Abstract:The effects of a mineral block for horses on in vivo digestibility and in vitro fermentability with equine fecal inoculum were evaluated. Fifty healthy horses from three groups (lactating mares n = 19, working horses n = 18, and maintenance horses n = 13) were randomly assigned to two treatment groups (with or without the mineral block; Ca 10.0%, P 12.0%, Zn 12.1 mg/kg, Cu 2,050 mg/kg, Mn 4,050 mg/kg, Se 30 mg/kg, and I 105 mg/kg). Dry matter digestibility was estimated with an internal marker. Samples of diet were incubated with equine fecal bacteria with varying amounts of mineral block (0, 1.1, 3.6, and 6.2 mg/g dry matter DM]) to record gas production and to estimate in vitro DM digestibility. The results showed that mineral supplementation with the blocks increased in vivo DM digestibility (P < .01) in all groups, but there was an interaction (P < .01) with a greater response in the maintenance horses (55.5% vs. 78.0%) compared to lactating mares (62.8% vs. 79.6%) and working (70.3% vs. 75.1%). Block consumption was lowest in the lactating mares (12.8 g/d), intermediate in the working horses (44.6 g/d), and highest in the maintenance horses (74.2 g/d). The mineral supplementation did not affect the kinetics of gas production but tended (P = .10) to improve the in vitro DM digestibility (37.01% vs. 38.34%). Mineral block supplementation increased dry matter digestibility in horses. The unsupplemented control diet was deficient in several minerals, and block intake was not proportional to the mineral requirements.
Keywords:Horse  Mineral lick block  Digestibility
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