First Report of Molecular Characterization of Argentine Isolates of Streptococcus equi subsp. equi by Pulsed-Field Gel Electrophoresis |
| |
| Institution: | 1. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Enfermedades Infecciosas, Buenos Aires, Argentina;2. Universidad Complutense de Madrid, Facultad de Veterinaria, Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Madrid, Spain;1. Children''s Medical Center, Tehran University of Medical Sciences, Tehran, Iran;2. Research Center of Pediatric Infectious Diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran;1. Veterinary Surgery and Radiology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, India;2. Division of Veterinary Surgery and Radiology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu-180009, India;1. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;2. Department of Pathobiology, Biotechnology Section, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;1. Department of Biological Sciences, School of Environmental and Health Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, South Africa;2. Food Security and Safety Niche Area, Faculty of Agriculture, Science and Technology, North-West University, Mmabatho, Mafikeng 2735, South Africa |
| |
| Abstract: | Strangles is one of the most frequently diagnosed equine respiratory infectious diseases in the world. It is caused by Streptococcus equi subsp. equi (S. equi), and it is an acute infection characterized by pyrexia, nasal discharge, pharyngitis, and abscessation of lymph nodes. Frequently, healthy horses might continue to harbor S. equi after clinical recovery. Although the genetic distance between S. equi isolates is short, strains can be differentiated by pulsed-field gel electrophoresis (PFGE) and single locus sequence typing for epidemiological studies. The aim of this study was to characterize by PFGE Argentine isolates of S. equi obtained from horses with acute strangles and those that had recovered. Bacterial isolation and identification of 80 S. equi isolates by phenotypic and genotypic tests were performed using samples from 29 horses with acute strangles and 95 from healthy animals. Also, the isolates were characterized by PFGE using Bsp120I and SmaI. Visual comparison of macrorestriction patterns generated with both enzymes revealed three different DNA fragment profiles with variations of one or two bands. Interestingly, an identical profile was found in isolates from the same horse and from horses that were infected at the same time, and the horses recovered from strangles continue to carry the same strain. Some vaccinated horses have been mild infected for a different strain from that of carriers suggesting other source of infection. This is the first molecular characterization of Argentine isolates of S. equi, which shows the presence of three strains between 2010 and 2013 in Buenos Aires. |
| |
| Keywords: | Strangles PFGE Epidemiology |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
