豌豆蚜防控的关键RNAi靶标基因筛选与分析

为筛选防控豌豆蚜Acyrthosiphon pisum的关键RNA干扰(RNA interference,RNAi)靶标基因，通过构建精氨酸激酶(arginine kinase,AK)、超氧化物歧化酶(superoxide dismutase,SOD)和几丁质合成酶(chitin synthase,CHS)这3个与豌豆蚜生长发育相关基因的dsRNA，于室内检测ds AK、ds SOD和ds CHS处理后对其致死效果，并比较4种dsRNA递送方式对其致死效果的影响，筛选获得防治效果明显的dsRNA及其递送方式。结果显示，处理48～168 h后，不同基因dsRNA处理组豌豆蚜的死亡率比阴性对照组高2.19倍～4.39倍，其中ds AK处理组豌豆蚜的死亡率为27.7%～91.1%，显著高于ds SOD、ds CHS处理组及阴性对照组。使用ds AK通过人工饲喂法和叶片注射饲喂法处理豌豆蚜，48 h即可出现显著致死效果。ds AK通过人工饲喂方法处理72 h后，豌豆蚜AK基因表达量比空白对照组降低43.2%，同时可抑制豌豆蚜的生长发育，使其体长比阴性对照组减少了5.9%。表明ds AK对豌豆蚜...

Screening and analysis of the pivotal RNAi target genes for the control of pea aphid Acyrthosiphon pisum

To screen for the key RNA interference (RNAi) target genes for controlling pea aphid Acyrthosiphon pisum, the dsRNAs targeting three genes associated with the growth and development of A. pisum, namely arginine kinase (AK), superoxide dismutase (SOD), and chitin synthase (CHS), were constructed. By comparing the lethal effects of different dsRNA (dsAK, dsSOD, and dsCHS) treatments on A. pisum, and the effects of four different dsRNA delivery methods on the lethal effects of aphids, an optimized A. pisum control method was proposed. The results showed that after 48-168 h, the mortality rate of aphids in the treatment group was 2.19-4.39 times higher compared to the negative control group. The lethal rate of dsAK to aphids was 27.7%-91.13%, significantly higher than that those of dsSOD, dsCHS treatment groups and the negative control group. Significant mortality effects on A. pisum could be observed within 48 h after dsAK treatment through artificial diet feeding or feeding with leaves injected with dsRNA solution. At 72 h after treatment with dsAK through artificial diet feeding, the expression of AK gene in A. pisum was reduced by 43.2% compared with blank control, while it also suppressed the growth and development of aphids, resulting in a 5.9% decrease in body length compared to the negative control. The results indicated that dsAK exhibited a significant lethal effect on pea aphids, showing promising prospects for its application in environment-friendly aphid prevention and control.