| 唐菖蒲组培脱毒技术研究 |
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| 引用本文： | 李昌禹,郭太君,焦培娟,张雅凤,杜令阁. 唐菖蒲组培脱毒技术研究[J]. 园艺学报, 2003, 30(3): 358-360 |
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| 作者姓名： | 李昌禹 郭太君 焦培娟 张雅凤 杜令阁 |
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| 作者单位： | (中国农业科学院特产研究所，吉林132109) |
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| 摘 要： | 在MS培养基上，附加5 mg/L病毒唑培养唐菖蒲，再经38~40 ℃热处理，切取微茎尖两次，去除了危害唐菖蒲的3种主要病毒TMV、CMV和TVY。以MS+ BA 0.5 mg/L+ NAA 0.5 mg／L+KT l mg/L为继代培养基，1/2MS无激素培养基为成球培养基，进行快繁获得了脱毒种球，移植后，植株生长发育状况得到明显改善。
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| 关 键 词： | 唐菖蒲 脱毒 |
| 文章编号： | 0513-353X(2003)03-0358-03 |
| 收稿时间： | 2003-01-05 |
| 修稿时间： | 2002-08-06 |
Studies on Virus-free and Rapid Reproduction in Cultural Plant of Gladiolus and Its Effect |
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| Li Changyu,Guo Taijun,Jiao Peijuan,Zhang Yafeng,and Du Lingge. Studies on Virus-free and Rapid Reproduction in Cultural Plant of Gladiolus and Its Effect[J]. Acta Horticulturae Sinica, 2003, 30(3): 358-360 |
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| Authors: | Li Changyu Guo Taijun Jiao Peijuan Zhang Yafeng and Du Lingge |
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| Affiliation: | (Institute of Special Economic Animal and Plant, Chinese Academy of Agricultural Sciences，Jilin 132109，China) |
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| Abstract: | A study on tissue culture of gladiolus hybridus of ‘Chenghongjiao’ and ‘Ziyinghua’ was carried out in MS medium with diferent phyto-hormone BA，KT and NAA．A MS medium supplemented with BA 0.5 mg/L+NAA 0.5 mg/L+KT 1 mg/L was for the regenerated and 1/2MS medium without hormone was for bulbs forming．The three main viruses of TMV，CMV and TVY in gladiolus were removed from cultural plant of gladiolus in the regenerated medium through 5-20 mg／L concentration of ribavirin，treatment of high temperature 38-40℃and 2 times of meristem-tip culture． |
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| Keywords: | Gladiolus Virus-free Effect |
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