新疆南疆猪繁殖与呼吸综合征病毒ORF5基因克隆与序列分析

[目的]掌握新疆南疆PRRSV流行毒株特点,为新疆南疆PRRS的有效预防和控制提供理论依据.[方法]研究利用克隆和测序获得了8个新疆南疆PRRSV ORF5基因全序列,并进行序列分析.[结果]8个新疆南疆PRRSV ORF5基因长度均为603 bp,它们之间核苷酸同源性为87.4; ～ 100.0;；与欧洲型代表株LV、美洲型代表株ATCC VR-2332、中国代表株CH-1a和中国HP-PRRS代表株JXA1核苷酸同源性分别为60.9;～63.5;、88.4;～ 89.9;、88.9; ～ 94.7;和87.4;～98.7;；与中国猪养殖场常用的几种弱毒疫苗株核苷酸同源性为87.4;～94.7;；与美洲型毒株和欧洲型毒株氨基酸同源性为84.5;～98.0;和50.0;～59.5;.[结论]研究的8株新疆南疆PRRSV株均属于美洲型亚群Ⅱ毒株.利用新疆南疆PRRSV分子流行病学调查结论,可为新疆南疆PRRS防制疫苗的选择等做迸一步的调整或指导奠定基础.

The Cloning and the Sequence Analysis of Southern Xinjiang Strain ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus

【Objective】This project aims to grasp the characteristics of pandemic strain of the Southern Xinjiang PRRSV and provide a theoretical basis for the effective prevention and control of the virus.【Method】In this study,eight complete sequences of 0RF5 genes of the South Xinjiang PRRSV were obtained by cloning and sequence analysis.【Result】The gene length of eight complete sequences of 0RF5 genes of the Southern Xinjiang PRRSV were all 603 bp,nucleotide homology between them was 87.4%to 100.0%;The nucleotide homology of ORF5 genes were 60.9%to 63.5%,88.4%to 89.9%,88.9%to 94.7%,and 87.4%to 98.7%,respectively,compared with LV of Europe type representative strain,ATCC VR -2332 of American type representative strain,CH - la of China type representative strain,and JXAI of the HP - PRRS China type representative strains.And compared with attenuated vaccine strains used wildly in China pig farms,their nucleotide homology was 87.4%to 94.1%.The amino acids homology of ORF5 genes compared with the American type representative strain and Europe type strain was 84.5%to 98.0%and 50.0%to 59.5%.【Conclusion】The five ORF5 genes of the Southern Xinjiang PRRSV all belong to the American type subgroup II strains.The molecular epidemiology investigation result of the Southern Xinjiang PRRSV has laid the foundation for the further adjustment and guidance in the choice of vaccine for Southern Xinjiang PRRS.